摘要
目的 研究阿魏酸钠 (SF)对大鼠结肠炎的抗炎保护作用及其机制。方法 建立大鼠乙酸性结肠炎模型。SF与5 氨基水杨酸灌肠给药 1wk后评价大鼠结肠黏膜损伤指数(CMDI) ,采用试剂盒及免疫组化方法检测结肠组织NO、MPO、PGE2 含量及结构型一氧化氮合酶 (cNOS) ,诱生型一氧化氮合酶 (iNOS) ,环氧合酶 1(COX 1) ,环氧合酶 2 (COX 2 )和核因子 κBp6 5 (NF κBp6 5 )的表达水平。 结果 SF(2 0 0、4 0 0、80 0mg·kg-1)灌肠用药均降低模型组大鼠升高的CMDI及结肠组织NO、MPO、PGE2 含量 ,下调iNOS、COX 2、NF κBp6 5的过度表达 ,亦抑制cNOS的正常表达水平 ,对COX 1表达影响不明显。SF用药呈现一定量效关系。结论 SF为一氧化氮合酶及部分选择性COX 2抑制剂 ,对大鼠结肠炎具有一定抗炎保护作用。
AIM To investigate the anti-inflammation protective effects of sodium ferulate (SF) on colitis rats and its mechanism. METHODS The colitis model of rats was produced by intracolon enema with acetic acid. SF and 5-ASA were used intracolonically for a week. The colon mucosadamage index (CMDI) was evaluated. Nitric oxide (NO), myelopexoxidase (MPO), prostaglandin (PGE_2), and the levels of expression of constitutive nitric oxide synthase(cNOS), induce nitric oxide synthase (iNOS), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and nuclear factor-kappaBp65(NF-κBp65) in the rats colon were detected by corresponding kits and immunohistochemical technology. RESULTS SF (200,400,800 mg·kg -1 ) can decrease the extents of CMDI and the levels of NO, MPO, PGE_2, the expression of cNOS, iNOS, COX-2, and NF-κBp65 in model group in a dose-dependent manner while the expression of COX-1 changes littlely. CONCLUSION SF is a NOS and partial selective COX-2 inhibitor and show therapeutic effect on colitis in rats.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第5期571-574,共4页
Chinese Pharmacological Bulletin
基金
湖北省科技攻关项目
No 2 0 0 1AA3 0 8B
关键词
阿魏酸钠
结肠炎
一氧化氮合酶
环氧合酶
sodium ferulate
colitis
nitric oxide synthase
cyclooxygenase
