摘要
目的:研究基质金属蛋白酶9(MMP-9)及其抑制剂(TIMP-1)在人肺鳞癌中的表达与转移、预后的相关性,探讨其在肺癌发生发展、侵袭和转移中的机制。方法:应用免疫组化S-P法检测30例有转移的肺鳞癌组织、20例无转移的肺鳞癌组织、15例正常肺组织、12例增生上皮组织、10例不典型增生组织中MMP-9、TIMP-1蛋白的表达。结果:正常粘膜上皮→增生上皮→不典型增生→无转移鳞癌→有转移鳞癌的演进过程中,两种蛋白表达率均呈上调趋势。MMP-9的表达在有转移癌与无转移癌、增生+不典型增生上皮与正常粘膜上皮之间,差异具有显著性(P<0.05)。鳞癌与正常上皮、增生上皮相比,MMP-9和TIMP-1的表达升高具有显著性(分别P<0.005,P<0.025;P=0.014,P<0.05)。两种蛋白的表达均随着鳞癌TNM分期的增加而上调,分期越晚表达率越高(P<0.01)。术后生存期<2年的肺癌患者与术后生存期≥2年者相比,MMP-9和TIMP-1表达的升高具有显著性(P<0.025,P<0.05),高表达者(84.6%,61.5%)较低表达者(50.0%,25.0%)生存期短,预后差。结论:肿瘤的发生发展、浸润转移等与细胞外基质过度降解、基底膜的破坏等密切相关。MMP-9阳性表达上调可以出现在癌前病变及肺癌的早期阶段,MMP-9基因的激活可能是肺癌癌变的重要因素;MMP-9。
Objective:To investigate express io ns of Matrix metalloproteinase-9(MMP9)and its inhibitor(TIMP1)and their correlation with metastasis and prognosis in lung squamous carcinoma.Methods:I mmunohistochemical streptaridin-perioxidase(S-P)method was used to ex-am in e protein expressions of MMP-9?TIMP-1on30metastasis lung squamous carcino ma,20no-metastasis squamous carcinoma ,15normal and12hyperplasia,10d ysplasia epithelium of control group.Results:In the developmemt of normal tis sue→hyperplasia→dysplasia→no-metasta-sis→metastasis squamous carcinoma,th e positive overexpression rates of MMP-9and TIMP pro teins all showed rising trend.The difference of MMP9expression in metastasis carcinoma versus no-met astasis carcinoma ,and hyperplasia+dysplasia epithelium versus normal group we re statisti cally significant (P<0.05).Comparing squamous carcinomar wit h normal group and hyperplasia epithelium, the elevation of MMP9and TIMP1e xpression were also significant (P<0.0 05,P<0.025;P=0.014,P<0.05respectively).Expressions of MM P9and TIMP-1have all positive relation with TNM grade,rising with TNM grade increasing(P<0.01).The difference of MMP9and TIMP1expression rates in patients of postoperative survive time <2year versus≥2year had statistical s ignificance(P<0.025,P<0.05),patients of higher expression rates( 84.6%,61.5%)had shorter survive time and more unfavourable prognosi s,compared to ones of lower expression rates(50.0%,25.0%).Conclu-sions:The genesis,development,invasion and metastsis of lung neoplasm had c lose relationship with the excess degradation and disruption of basement membra nce et al.activation of MMP-9gene is an important factor of oncogenesis of l ung carcinoma,MMP-9and TIMP-1may play an important role in lung carcinoma invasion and metastasis,their overexpression could act as a refevence stan dar d to evaluate metastasis and unfavourable prognosis of lung carcinoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2003年第5期305-309,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:39870305)
湖北省教委重点课题基金(编号:97A050)
关键词
肺肿瘤
免疫组织化学
基质金属蛋白酶类
肿瘤转移
肿瘤预后
Lung neoplasms Immunohistochemical Matrix metalloproteinases Neoplasms metas tasis Neoplasms prognosis
