摘要
探讨ｐ５３和ｒａｓ ｐ２１蛋白的表达与眼睑肿瘤临床预后的关系。方法 采用免疫组化ＳＡＢＣ法检测了９８例眼睑恶性肿瘤组织中ｐ５３和ｒａｓ ｐ２１蛋白的表达。结果 在鳞状细胞癌旁正常及轻度不典型增生上皮中未见ｐ５３和ｒａｓ ｐ２１蛋白表达；而中、重度不典型增生，原位癌，浸润癌ｐ５３和ｒａｓ ｐ２１蛋白阳性表达率逐渐增高。其中基底细胞癌ｐ５３和ｒａｓ ｐ２１阳性表达率分别为５０．０％，５２．５％；鳞状细胞癌分别为５４．３％，６２．９％；睑板腺癌分别为５６．５％，６５．２％。随着肿瘤分化程度的降低，ｐ５３和ｒａｓ ｐ２１蛋白阳性表达率及阳性表达强度逐渐增高；ＳＣＣ中ｐ５３及ｒａｓ ｐ２１蛋白阳性表达与组织分化程度之间呈显著负相关，且与肿瘤淋巴转移有关。结论 ｐ５３和ｒａｓ ｐ２１的表达可作为判断眼睑肿瘤恶性程度、淋巴结转移的重要指标。
Objective To study the expression of p53 and p21 protein in cutaneous eyelid tumors and the correlation with clinical prognosis. Methods The expression of p53 and ras p21 protein was examined by SABC immunohistochemical technique in 98 cutaneous eyelid tumors. Results Although expression of p53 and ras p21 protein wasn't detected in adjacent epithelium and mild dysplasias, the positive rate of p53 and ras p21 increase by degrees in moderate dysplasias, severe dysplasias, carcinoma in situ and invasive tumors. The positive rate and intensity of p53 and ras p21 protein immunostaining increase with the decreasing degree of histological differentiation. The positive intensity of p53 and ras p21 protein was associated with histological differentiation of SCC,and have a well correlation with recurrence and metastasis. Conclusion The expression of p53 and ras p21 have been shown to be the significant predictors for clinical prognosis of cutaneous eyelid tumors.
出处
《眼科研究》
CSCD
北大核心
2001年第5期439-442,共4页
Chinese Ophthalmic Research
基金
山东省卫生厅基金资助项目(基金编号:1999CA1CKA4)
