期刊文献+

DNA生物传感器在“基因诊断”中的实验研究 被引量:2

The Research of DNA Biosensor for Gene Diagnoses
在线阅读 下载PDF
导出
摘要 为了建立石英晶体DNA传感器检测乙肝病毒基因的方法,在石英晶体金表面固定单链DNA,构成压电晶体HBV DNA生物传感器,与待测样品进行杂交反应。结果DNA传感器能准确的诊断血清中的HBV病毒基因。石英晶体HBV DNA生物传感器具有操作省时,简单,能用于临床实验、诊断、检测。 In order to construct a new type of piezoelectric quartz crystal DNA biosensor for HBV, the single-chain DNA sequence is immobilized on the surface of gilt quartz crystal to make a HBV gene sensor, then to hybridize on the gene sensor in the serum sample. The quartz crystal DNA biosensor is able to detect the HBV exactly in the real HBV serum sample. The piezoelectric quartz crystal DNA biosensor is a fast dignostic tool with easy operation for detecting HBV in clinical laboratories.
出处 《中国医疗器械杂志》 CAS 2004年第1期37-39,共3页 Chinese Journal of Medical Instrumentation
基金 广东省重点攻关课题[计划编号:2KMO4301S] 广州市重点攻关课题[计划编号:2000-Z-028-01]
关键词 DNA生物传感器 基因诊断 实验研究 石英晶体 DNA biosensor, quartz crystal, diagnosis
  • 相关文献

参考文献4

  • 1Frank Caruso, et al. Quartz crystal microbalance study of DNA immobilization and hybridization for nucleic acid sensor development, Anal. Chem. 1997, 69:2043-2049.
  • 2Yoshio Okahata, et al. Kinetic measurements of DNA hybridization on an oligonucleotide-immobilized 27-MHz quartz crystal microbalance. Anal. Chem.,1998, 70:1288-1296.
  • 3Koji Hashimoto, et al. Microfabricated disposable DNA sensor for detection of hepatitis B virus DNA. Sensors and Actuators B. 1998, 46:429-430.
  • 4Sara Tom belli,et al. Coupling of a DNA piezoelectric biosensor and polymerase chain reaction to detect apolipoprotein E polymorphosis. Bio sensors & Bioelectronics. 2000, 15: 363-370.

同被引文献18

  • 1叶学松,徐成华,樊海涛,王平,汪元美.一种新型的声表面波液相检测系统的研究[J].浙江大学学报(工学版),2004,38(8):1051-1055. 被引量:7
  • 2陆斌,韦钰.基子全内反射萤光原理的免疫传感器[J].中国生物医学工程学报,1993,12(2):142-148. 被引量:26
  • 3RAPP M,REIBEL J,VOIGT A,et al.New miniaturized SAW-sensor array for organic gas detection driven by multiplexed oscillators[J].Sensors and Actuators B,2000,65 (1 -3):169-172.
  • 4HUANG X J,MENG F L,PI Z X,et al.Gas sensing behavior of a single tin dioxide sensor under dynamic temperature modulation[J].Sensors and Actuators B,2004,99 (2 -3):444 -450.
  • 5DARINSKII A N,BIRYUKOV S V,WEIHNACH M.Fundamental frequency degeneracy of standing surface acoustic waves under metallic gratings on piezoelectric substrates[J].J Acoust Soc Am,2002,112 (5 Pt 1):2003 -2013.
  • 6GAMBLE K J,MALOCHA D C.Simulation of short LSAW transducers including electrode mass loading and finite finger resistance[J].IEEE Trans Ultrason Ferroelectr Freq Control,2002,49 (1):47-56.
  • 7NAKAMURA H,KARUBE I.Current research activity in biosensors[J].Anal Bioanal Chem,2003,377 (3):446 -468.
  • 8KWON Y,ROH Y.Development of SH-SAW sensors for underwater measurement[J].Ultrasonics,2004,42 (1-9):409-411.
  • 9Chen M,Liu M,Yu L,et al.Construction of a novel peptide nucleic acid piezoelectric gene sensor microarray detection system[J].Nanosci Nanotechnol,2005,8(5):6-10.
  • 10Huang X,Meng F,Pi Z,et al.Gas sensing behavior of a single tin dioxide sensor under dynamic temperature modulation[J].Sens Actuators,2004,99(2-3):444-450.

引证文献2

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部