摘要
目的 研究慢性乙型肝炎患者服用拉米夫定后乙肝病毒YMDD变异情况 ,同时探索引起YMDD变异的影响因素。方法 选取 136例应用拉米夫定治疗的慢性乙型肝炎患者 ,ELISA检测HBeAg ,抗HBe ;PCR检测HBVDNA浓度 ,通过HBVcodon 5 5 0特异性PCR扩增确定YVDD及YIDD变异情况。结果 79例疗程达 5 2周者出现变异 12例 ,同时YVDD及YIDD变异 2例 (2 5 % ) ,5 7例疗程达 10 4周者出现变异 16例 ,同时YVDD及YIDD变异 4例。治疗前HBeAg阳性 82例中YMDD变异 17例 ,抗HBe阳性 5 4例中YMDD变异 17例。结论 拉米夫定引起YVDD及YIDD变异是耐药的原因 ,YMDD变异与发生HBV前C区变异无关系 。
Objective A research on the chronic hepatitis B patients who have used Lamivudine was carried out to observe there HBV YMDD variation and at the same time to probe the influenced factors that lead to YMDD variation.Methods 136 patients who have used lamivudine to cure their chronic hapatitis B was picked up.ELISA was used to examine HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc,used PCR to examine HBV DNA's dentity and YVDD and YIDD's variation.Results During 79 patients whose treatment course have reached 52 weeks,there are 12 patients appear variation and 2 patients appear YVDD and YIDD's variation simultaneously(2 5%).16 patients appear variation and 4 patients appears YVDD and YIDD's variation simultaneously,during 57 patients whose treatment course have reached 104 week.During 82 patients whose HBeAg are positively and 54 patients whose anti-HBe are positively before treatment,there are 17 and 11 patients appear YMDD variation after treatment,respectively.Conclusion YVDD and YIDD's variation caused by lamivudine is due to its drug-resisting YMDD's variation has nothing to do with HBV variation in Pre C,but has something to do with HBV DNA density before treatment.
出处
《医药论坛杂志》
2003年第10期6-7,共2页
Journal of Medical Forum
