摘要
为了探索桉树抗青枯病树种的无性系快速筛选和繁殖技术,配制了3种类型的培养基,采用组培方法对桉树愈伤组织的抗病性和桉树无性系的快速繁殖方法进行了研究。结果表明:诱导愈伤组织形成的以MS+6-BA0.25mg/L+NAA0.5mg/L培养基最好;对愈伤组织芽的分化,最好的培养基是MS+6-BA0.25mg/L+NAA5mg/L;诱导生根以White+IBA0.5mg/L+2%蔗糖培养基为最佳;分别对桉树愈伤组织刺伤、幼苗伤根、幼苗截顶后接种青枯病,并测定它们的抗病性,其测定结果相同,说明可用桉树愈伤组织筛选抗青枯病树种。
This paper deals with the rapid sieve selection of Eucalyptus tree species and the quick-propagation of Eucalyptus clones, with three kinds of culture mediums, by means of tissue culture. The findings show that MS+6-BA 0.25 mg/L+NAA 0.5 mg/L is the best culture medium in the callus tissue forming; MS+6-BA 0.25 mg/L+NAA 5 mg/L is the best culture medium in callus bud differentiation; white+IBA 0.5 mg/L+2% sucrose is the best in the callus seedling rooting; the effects of using the stabbed callus inoculation and, the seedlings immersed in bacterial liquid after injuring their roots and, the seedlings dropped with bacterial liquid after cutting the shoot tips on the tested Eucalyptus's resistance to Pseudomonas solanacearum are much the same. The studying results indicate that it is feasible for us to select the Pseudomonas solanacearum resistance tree species with Eucalyptus tissue culture.
出处
《中南林学院学报》
CSCD
2003年第6期117-120,共4页
Journal of Central South Forestry University
关键词
林学
桉树
愈伤组织
抗青枯病
树种
forestry
Eucalyptus
callus
resistance to bacterial disease
tree species
