摘要
目的 探讨肥大细胞(Mast cell,MC)在肾组织中的浸润与肾间质损害、转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)表达及肾功能的关系。方法 收集46例不同病理类型的肾小球肾炎患者的临床资料和肾活检标本,采用免疫组织化学技术检测MC在肾组织中的浸润及TGF-β1、α-SMA的表达,并分析它们之间及其与肾间质损害、肾功能的相关性。结果 正常肾组织中偶有或无MC的存在,而在患肾的间质中则发现大量MC浸润,主要分布在皮质,少数在髓质,其分布与间质中α-SMA阳性细胞的分布大体一致。MC的数量与肾间质损害程度、TGF-β1、α-SMA的表达、Scr浓度呈正相关(r分别为0.49、0.90、0.94、0.71,P均<0.001);与Ccr呈负相关(r=-0.60,P<0.0001)。MC数随肾间质损害程度的加重明显增加(P<0.01)。结论 MC数与肾间质损害程度呈正相关。MC可能是肾间质损害的重要参与者。
Objective To investigate the correlation of number of mast cells in the interstitium with tubulointerstitial lesions,renal function,expression of TGF-β1 and α-SMA. Methods The intensity of mast cell infiltration and expression of TGF-β1 and α-SMA in renal biopsies from 46 patients with different nephritides were studied by immunohistochemistry,using a monoclonal anti-human mast cell tryptase antibody,polyclonal anti-human TGF-β1 and monoclonal anti-human α-SMA antibody. Clinical data of these 46 patients at the time of renal biopy were collected. Results Mast cells were scarce or absent in normal kidney,but large numbers were present in the renal interstium of the diseased kidneys. The distribution of mast cells was mainly cortical with few medullary infiltrating cells. This distribution was concordant with that of α-SMA-positive myofibroblasts. Mast cells were correlated with interstitial lesions (r = 0. 90,P < 0. 0001),expression of TGF-β1 ( r =0. 49,P<0.001),α-SMA positive cells (r=0. 94,P < 0. 0001),
serum creatinine ( r =0. 71,P < 0. 0001) and creatinine clearance rate ( r = - 0. 60,P < 0. 0001). With aggravation of interstitial lesions degree,the number of tryptase-positive mast cells had increased obviously (P < 0. 01) . Conclusion Increased infiltration of mast cells correlates with the degree of interstitial lesions. This suggests that mast cells may play an important role in the development of renal fibrosis.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2003年第5期286-291,共6页
Chinese Journal of Nephrology
关键词
肥大细胞
慢性肾小球肾炎
肾间质损害
化生长因子β
Glomerulonephritis
Mast cell
Transforming growth factor beta 1
Tubulointerstitial lesions
