摘要
目的 探讨室间隔缺损的实验动物模型的建立方法。方法 15只幼猪在全麻下经右心耳行室间隔穿刺造口术。术后 3天行超声心动图检查 ,测定最大分流速率。术后 1个月行心导管检查 ,测定肺 -体循环血流比 (Qp Qs)。结果 有 9只猪建模成功。超声心动图示最大分流流速Vmax为 1.7～ 4 .4m s。血流动力学指标测定结果示肺动脉压无显著升高 ,Qp Qs1.6 8～ 2 .12 ,平均 1.84± 0 .2 4。结论 采用改良的Synhorst方法成功地建立了幼龄室间隔缺损动物模型。
Objective To investigate the method to establish experimental animal model of ventricular septal defect (VSD) in pigs. Methods Thoracotomy was performed on 15 young pigs under general anaesthesia. A spool-like ring amounted on a specially designed centesis tool was put into right ventricle via an incision on right auricle which was pushed to puncture through the septum. The ring was then left on the septum to create the artificial VSD. 3 days after the operation, echocardiography was conducted to verify the existence of VSDs. The animals were catheterized one month later to obtain hemodynamic data. Results 9 pigs survived the operation with successful acquisition of VSDs. Doppler echocardiography demonstrated the transseptal shunts with peak velocities ranged from 1.7-4.4m/sec. Cardiac catheterization showed the Qp/Qs of 1.68-2.12 although the pulmonary pressures remained in normal limits. Conclusion The authors report their experience in the establishment of experimental VSD model in pigs in revised Synhost method. The preliminary result shows that the VSD model in pigs is feasible and worth improving further in order to provide a useful animal model for the pathophysiological study of intracardiac left to right shunt in the future.
出处
《中国比较医学杂志》
CAS
2003年第4期242-245,共4页
Chinese Journal of Comparative Medicine
基金
四川大学青年基金资助 ( 4 3 2 0 0 3 )
