摘要
目的:通过外科方法建立膜部/干下室间隔缺损(VSD)的动物模型。方法:6只成年草狗在全麻和气管插管下,左侧垫高45度卧位,经右侧第四肋间进胸,在胸腔内心超大血管短轴定位后,用改良的打孔器进行穿刺,经心超检查证实和测定最大分流速度和压力。结果:3只狗建模成功,2只建立了膜部室缺的模型,1只为干下室缺,超声心动图示室间隔缺损直径1.8～3.6mm,最大分流速度1.8～4.0m/s,跨室缺压差为42～51mmHg,肺动脉压力为25～29mmHg。结论:采用改良的主动脉大隐静脉近端吻合器打孔器在心超定位后用外科手术方法成功建立狗膜部/干下VSD动物模型。
Objective： To establish the experimental animal models of sub-arterial infundibular/membranous ventricular septal defect （VSD） in dogs. Methods： Thoracotomy was performed on six dogs at right fourth costal region and left lateral decubitus was padded 45 degree high under general anesthesia and intrabronchial intubation.Revised aortic cutter were put into right ventricle via an incision on right ventricular outflow, and vertically punctured through the septum after intrathoracic echocardiographic examination. VSDs were determined by echocardiography and the transseptal shunt Was measured. Results： Three dogs survived the operation with successful acquisition of VSDs, among which two showed membranous VSDs, one sub-arterial infundibular VSD. One week later, Doppler echocardiography demonstrated the defect diameter varied from 1.8-3.6 mm, the transseptal shunts with peak velocities ranged froml.8-4.0 ntis,the transseptal defect pressure ranged from 42-51 mmHg and the pulmonary pressure ranged from 25-29 mmHg. Conclusion： The successful animal models of sub-arterial infundibular/membranous VSDs are surgically establishd with revised aortic cutter after correct echocardiographic localization.
出处
《南京医科大学学报（自然科学版）》
CSCD
北大核心
2006年第3期189-191,F0004,共4页
Journal of Nanjing Medical University(Natural Sciences)
