摘要
采用5×5拉丁方设计,对5头大约克阉公猪(大约30kg)实施回盲肠瓣T型瘘管(PVCT)手术,用2,6 二氨基庚二酸(DAPA)标记法,测定以豆饼为基础的半纯合日粮中添加不同浓度的豌豆皮(4%、8%、12%)和纯稻壳(8%)对猪回肠微生物氮及微生物氨基酸流量的影响。结果表明:猪粪总氮中来源于微生物氮的量为74 53%～90 00%,回肠食糜氮中则为30 94%～50 26%;回肠食糜氨基酸流量中,有19 85%(脯氨酸)～51 42%(精氨酸)是来自微生物氨基酸;而微生物氨基酸在回肠内源性氨基酸流量中所占的比例较大,为30 49%(脯氨酸)～98 50%(亮氨酸)。豌豆皮与纯稻壳对猪回肠微生物的作用效果不尽相同,4%的豌豆皮与8%的纯稻壳的作用效果相似,而8%和12%的豌豆皮对猪回肠微生物的影响要高于8%的纯稻壳。
In this paper,the flow of bacterial nitrogen (BN) and bacterial amino acid were determined,respectively,using the microbial marker 2,6diaminopimelic acid(DAPA)method in soybean meal based diets with different level of the peahull(4%,8% and 12%)and the rice hull(8%)fed to growing pigs.Five Yorkshire barrows(about 30 kg BW)equipped with PVCT for collection of ileal digesta were fed one of the diets according to a 5×5 Latin square design.The experimental results showed that 7453% to 90% of the total nitrogen in feces and 3094% to 5026% of the total nitrogen in ileal digesta were of bacterial origin.The differences ranged from 1985(Proline)to 5142(Arginine)percentage units in the flow of each amino acid at the terminal ileum were bacterial amino acid.Whereas,the rate of bacterial contribution to endogenous amino acids flow in ileal digesta was great,and the different value ranged from 3049%(Proline)to 9850%(Leucine).When the effect on the flow of BN and bacterial amino acid in ileal digesta were compared between the diet of peahull and rice hull,4% peahull had a similar effect to 8% rice hull,but the effect of 8% or 12% peahull was greater than that of 8% rice hull.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2003年第3期239-245,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
中国科学院"百人计划"项目(猪内源性氨基酸排泄规律与基础代谢的关系研究)资助
关键词
日粮纤维
猪
回肠微生物氮
微生物氨基酸流量
影响因素
Pea-hull
Rice hull
Bacterial nitrogen(BN)
Bacterial amino acid
2,6-diaminopimelic acid(DAPA)
