摘要
从豫西地区发病鸡群中分离的鸡减蛋综合征病毒 (EDSV) ,经非免疫鸡胚增殖后 ,用差速离心法纯化和蛋白酶 K法抽提 ,得到全长约 34.3kb的 EDSV基因组。分别以 Hind 、Bam HI、Eco RI及 Bam HI+ Eco RI对 EDSV -g DNA进行限制性酶切 ,分别得到 10、4、4和 7个片段。用 10 g/ L琼脂糖凝胶电泳酶切样品并以凝胶成像系统对酶切片段的大小和分子量进行测定。将这些结果与 NE4、WPDV2 0 5、AV-12 7标准株及 B8/ 78株基因组 DNA由相同酶切的结果进行比较 ,发现豫西地区 EDSV与AV- 12 7和 B8/ 78较为相似 ,但也存在微小差别 。
Restriction endonuclease maps of egg drop syndrome virus(EDSV) isolated from the west region of Henan province were made by using four different restriction endonucleases HindⅢ, BanHI, EcoRIand BamHI+EcoRI. It was found that the DNA fragments were 9, 4, 4 and 7, respectively. Compared with NE4, WPDV205, AV 127 and B8/78, There were some differences in restriction maps and fragments. It was proven that different EDSV strain had different DNA types.
出处
《动物医学进展》
CSCD
2003年第3期100-102,共3页
Progress In Veterinary Medicine
基金
河南省自然科学基金项目(0 1 2 4 0 4 0 0 2 7)
