摘要
目的 :以指纹图谱层方式 ,研究补肾方分煎和合煎提取物中化学成分的差异。方法 :以常规方法制备补肾方分煎和合煎的提取物 ,采用HPLC法 ,考察并确定最佳的检测波长、不同品牌的C18填料的色谱柱 ,并采用优化的色谱条件对补肾方的合煎和分煎提取物进行测定 ,比较两者指纹图谱的差异。结果 :分煎和合煎提取物中化学成分组成存在差异 ,分煎提取物中主要化学成分的提取率大于合煎提取物 ,且共有化学成分的组成比例也不同。结论 :补肾方分煎和合煎提取物中主要化学成分组成及其共有成分比例存在差异。
AIM: To study on the differences of chemical compos i tion between extracts of Bushen recipe prepared by decocting together and single , with fingerprint technology. METHODS: The extracts of Bushen recipe were prepared by decocting together and single under the same process. The comparison was done under the different wavelength s and different brand columns, HPLC method determined the extract's fingerprint within the range of optimized conditions. RESULTS: The ratio of The compositions and its main chemical substa nces are the different between the two extracts, and the extract rate of the main chemical substances are higher in extrat decocted single than in extract decocted togethe r. CONCLUSION: There are some chemical substances and composition's ra tio differences in the extracts of Bushen recipe made by decocting together and single.
出处
《中成药》
CAS
CSCD
北大核心
2004年第1期7-9,共3页
Chinese Traditional Patent Medicine
基金
上海市科学技术委员会资助项目 (项目号 :0 0 4 31 92 2 6 )
关键词
补肾方
指纹图谱
分煎
合煎
HPLC
Bushen recipe
decoction together
decoction single
fingerprint
HPLC
