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吸氧预处理诱导的大鼠脑内Fos表达 被引量：1

Fos expression in rat brain induced by preconditioning with pure oxygen inhalation
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摘要 目的 :观察原癌基因c fos是否参与了对吸氧预处理诱导脑缺血耐受的产生 .方法 :随机将大鼠分为 2组 :吸氧预处理组和对照组 (每组n =6) .吸氧组大鼠吸纯氧 2 4h ,对照组大鼠吸 2 10mL·L-1O2 2 4h .2 4h后经心灌流、取脑 ;用免疫组化法观察Fos蛋白在大鼠脑内的表达 ,比较两组大鼠的表达差异 .结果 :Fos样免疫反应阳性神经元在脑内分布广泛 ,两组动物脑内的Fos阳性细胞的分布模式非常相似 .但吸氧预处理的大鼠脑内许多脑区Fos表达明显增多、增强 .结论 :吸氧预处理可激活脑内的一些核团 ;原癌基因c AIM: To investigate whether oncogene c fos is involved in the regulation of the brain ischemic tolerance induced by oxygen inhaling preconditioning. METHODS: SD rats were randomly divided into 2 groups: oxygen inhaling preconditioning group and control group ( n =6 each). The rats in oxygen inhaling preconditioning group inhaled Pure oxyen for 24 h, while the control animals inhaled 210 mL·L -1 Pure oxgen for 24 h. 24 h after the treatment, all the rats were perfused transcardially and the brains were moved out. The Fos expression in rat brain was detected by using ABC immunohistochemistry method and the difference between the two groups was studied. RESULTS: There was a wide spread expression of Fos like reactivity production and the distribution patterns in the two groups were similar. However, the Fos expression increased in oxygen inhaling preconditioning rats in the following brain areas: solitary tract nucleus, area postrema, lateral parabranchial nucleus, caudal part of spinal nucleus of 5th nerve, ventrol tegmental nucleus, periaqueductal nucleus, supramammillary nucleus, supraoptic nucleus, hippocampus, subiculum, posterolateral cortical amygdaloid nucleus, anterior cortical amygdaloid nucleus, lateral septal nucleus, bed nucleus of stria terminalis, globus pallidus, Calleja island, occipital cortex, retrosplenial cortex, parietal cortex, forelimb and hindlimb area of cortex, and frontal cortex. CONCLUSION: Some nuclei can be activated by oxygen inhaling preconditioning. Oncogene c fos is involved in the regulation of the brain ischemic tolerance induced by oxygen inhaling preconditioning.
作者 张西京 熊利泽 王曦 胡文能
机构地区 第四军医大学西京医院麻醉科 第四军医大学神经科学研究所
出处 《第四军医大学学报》 北大核心 2003年第7期610-613,共4页 Journal of the Fourth Military Medical University
基金 第四军医大学西京医院创新课题 (2 0 0 0 0 8)
关键词 氧疗 预处理 基因 FOS 大鼠 脑 oxygen therapy preconditioning gene, fos rats brain
分类号 R322.8 [医药卫生—人体解剖和组织胚胎学]
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第四军医大学学报
2003年 第7期

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