摘要
利用免疫细胞化学双标技术，研究了大鼠后脚掌注入ｆｏｒｍａｌｉｎ时脊髓ｃ－Ｆｏｓ蛋白和ＮＭＤＡ受体在脊髓灰质不同层次神经元的分布。结果表明，脊髓背角Ⅰ－Ⅱ层、Ⅴ－Ⅵ层、和Ｘ层中的Ｆｏｓ样免疫反应阳性神经元中分别有２８．６％，５８．１％和６４．８％呈现ＮＭＤＡ受体样免疫反应阳性。此结果提示长时间的外周伤害性刺激作用时，脊髓不同层次的伤害性感受神经元可能由于ＮＭＤＡ受体分布的不同而在持续痛刺激过程中起着不同的作用。
The coexistence of c Fos protein and NMDA receptors in the spinal cord following peripheral formalin injection into rat hindpaw was examined by using double immunocytochemical techniques.The results showed that about 26.8%,50.1%,and 64.8% of c Fos like immunoreactive (LI) neurons in laminae Ⅰ Ⅱ,laminae Ⅴ Ⅵ,and lamina Ⅹ of the dorsal horn were also NMDA like immunoreactive neurons,suggesting that the nociceptive neurons located in different spinal laminae might be functioning differentially during long lasting peripheral noxious stimulation,owing to the differential distribution of NMDA receptors on these neurons.
