摘要
目的 探讨昆明山海棠碱对淋巴瘤细胞核DNA的影响 ,以了解THH碱诱导细胞凋亡的机制。方法 THH碱诱导Jurkat细胞后 ,TUNEL荧光标记DNA链断裂 ,细胞DNA含量分析方法分析DNA片段化 ,均以流式细胞术检测。结果 THH碱能诱导Jurkat淋巴瘤细胞DNA链断裂 ,之后DNA发生片段化。结论 提示在THH碱诱导Jurkat凋亡过程中 。
Objective To investigate the effects of Tripterygium Hypoglaucum (Levl) Hutch alkaloids on the nuclear DNA strand breaks and DNA fragmantation in Jurkat T lymphoma cells to understand the mechanisms of the apoptosis. Methods After Jurkat cells were induced by the alkaloids, DNA stand breaks were labeled by TUNEL assay and DNA fragmentation was analyzed by DNA content analysis. Flow cytometry was performed to determine these phenomena. Results THH alkaloids could effectively induce DNA stand breaks and DNA fragmentation. Conclusion There are great changes in nuclear DNA in the apoptosis of Jurkat T lymphoma cells induced by THH alkaloids.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第17期1515-1517,共3页
Journal of Third Military Medical University
基金
德国联邦教育科学研究技术部 (BMBF)基金资助项目
国家自然科学基金资助项目 (C30 10 0 2 4 1)
第三军医大学中青年科研基金资助项目 ( 2 0 0 0 2 0 0 1)
