摘要
目的 :研究灵芝多糖对顺铂肾毒性的预防作用。方法 :♀Wistar大鼠随机分为生理盐水组、顺铂组、灵芝多糖组、灵芝多糖 +顺铂组 ,连续ip给药 5d后采样 ,分别测血肌酐 (Scr)、尿素氮 (BUN)、肾皮质组织丙二醛 (MDA)含量、超氧化物歧化酶 (SOD)活性 ,红细胞SOD活性和血清MDA含量 ,并观察肾脏形态结构的改变。结果 :顺铂组血清Scr、BUN明显升高 ,红细胞SOD活性降低 ,血清MDA含量升高 ;肾皮质组织MDA含量升高 ,SOD活性降低。灵芝多糖 +顺铂组血清Scr、BUN降低 ,红细胞SOD活性明显降低 ,血清MDA明显降低 ,肾皮质组织MDA含量降低 ,SOD活性升高 ;酶组化和病理切片显示肾脏结构较顺铂组明显改善。结论 :灵芝多糖可减轻顺铂肾毒性 ,其机制可能与抑制顺铂致血液和肾皮质脂质过氧化反应增强有关。
Objective: To study the preventive actions of ganoderma lucidum polysaccharide(GLP) against kidney damage induced by cisplatin. Methods : Female Wistar rats were randomly divided into normal saline(NS) group, cisplatin(CDDP) group, GLP group, CDDP+GLP group. The changes of Scr, BUN, MDA, SOD were measured and renal structure was observed after 5 days by injecting drugs. Results : The contents of serum Scr and BUN of CDDP group were significantly highter than that of NS group. The activity of RBC SOD reduced and the contents of serum MDA increased. The contents of renocortical tissue MDA increased and the activity of SOD declined in renocortical tissue. The contents of serum Scr and BUN of GLP+CDDP group were significantly lower than that of CDDP group. The activity of RBC SOD increased and the contents of serum MDA declined. The concents of renocortical tissue MDA declined and the activity of SOD increased in renocortical tissue. The pathological slice indicated that renal structure was significantly improved. Conclusion : GLP may reduce cisplatin nephrotoxicity and its mechanism may be correlative with that GLP inhibited the blood and renocortical tissue lipid peroxidation increasing.
出处
《中成药》
CAS
CSCD
北大核心
2003年第12期990-993,共4页
Chinese Traditional Patent Medicine
关键词
灵芝多糖
顺铂
肾皮质
脂质过氧化
大鼠
ganoderma lucidum polysaccharide
cisplatin
renocortical tissue
lipid peroxidation
rat
