摘要
取胃体大弯的胃粘膜上皮细胞在RPMI164培养液中,加成年雄性猪颌下腺提取液(PEGF)及成年雄性小鼠颌下腺提取液(M60EGF),用I^(125)UdR掺入方法测定细胞DNA合成。结果:盐水对照组为4168±171.5cpm,PEGF组为21.980±997.8cpm(P<0.0001),M60 EGF组为6132±262.7cpm(P<0.01)。鼠EGF加热80℃对胃粘膜DNA合成无效应,提示鼠EGF不耐热。实验结果表明:ECF是胃十二指肠粘膜的营养因子,对胃粘膜上皮细胞的生长有促进作用。
The gastric mucosal epithelial cells on. the greater curvature of the stomach were cultured in RPMI 1640 media containing PEGF and M60 EGF which were extracted from the submaxillary gland of male adult pig and mice. The cellular DNA synthesis was determined with I125 UdR incorporation method. The results showed that the saline control group was 4168±171.5 cpm, PEGF group 21980±997.8 cpm ( P<0.0001 ) and M60 EGF group 6132±262.7 cpm ( P<0.01 ) . Mice EGF heated to 80℃. had no effect on the DNA synthesis of the gastric mucous membrane, which suggested mice EGF do not tolerate heat. The xperimental results indicated that EGF was a nutrient factor of gastroduodenal mucous membrane and could promot the function for the growth of the gastric mucosal epithelial cells.
出处
《温州医学院学报》
CAS
1992年第2期81-83,共3页
Journal of Wenzhou Medical College
关键词
胃粘膜
上皮细胞
表皮生长因子
gastric epithelial cell
epidermal growth factor
