摘要
用培养的布氏杆菌菌体,通过超声波裂解、反复离心制备出布氏杆菌细胞壁抗原。将细胞壁抗原作1:128稀释,用作牛种布氏杆菌酶联免疫吸附试验(ELISA)的抗原;将布氏杆菌细胞壁抗原作1∶32稀释,用作平板凝集试验抗原;把细胞壁抗原作1∶16稀释用作试管凝集试验抗原,分别建立了牛布氏杆菌ELISA试验、平板凝集试验和试管凝集试验。用这3种方法,检测已知200份平板凝集试验阴性血清,5份平板凝集试验阳性血清。结果5份阳性血清在平板凝集试验、试管凝集试验和ELISA试验中均为阳性;200份阴性血清在平板凝集试验和试管凝集试验中均为阴性,在ELISA试验中有1份为阳性。试验证明,细胞壁抗原,既能用于传统的平板凝集试验和试管凝集试验。
Brucella cell wall antigen was prepared by bacteria-culture, ultrasonic breakage and repeated centrifugation. The cell wall was diluted in 1:128 and used as ELISA antigen; and diluted in 1:32, as plate agglutination antigen; and diluted in 1:16, as SAT (serum agglutination text) antigen,thus establishing ELISA,plate agglutination text, and SAT for detection of bovine brucellosis. 200 negative serum and 5 positive serum in plate agglutination text were detected by the three texts. Result indicated that the 5 positive sera were all positive in plate agglutination text, SAT and ELISA; The 200 negative sera were negative in plate agglutination text and SAT,and one was positive in ELISA. It was shown that the cell wall antigen can be used as antigen in ELISA as well as in plate agglutination text SAT.
出处
《中国动物检疫》
CAS
2004年第2期24-26,共3页
China Animal Health Inspection
关键词
细胞壁
抗原
布氏杆菌病
诊断试验
牛
Bovine brucella, Cell wall antigen,Agglutination text,ELISA
