摘要
从患暴发性传染病的家养鲫鱼分离到嗜水气单胞菌,其培养物经硫酸铵沉淀,DEAE-纤维素层析及 Sephsdex G100凝胶过滤纯化,获得一种单一多肽的外毒素,分子量为52.5kd。该毒素对热敏感,对胰酶有抗性,最适 pH5—8,具有溶血性、肠毒性和细胞毒性,腹腔注射致死小白鼠和鲫鱼,其生物学活性可被同源抗毒素中和。鉴于该毒素的独特性质,建议命名为hec 毒素。
An extracellular toxin produced by Aeromonas hydrophila from cultured crucian carpwith septicemia was detected.The toxin was purified by ammonium sulfate pricipitation,DE-AE-cellulose chromatography and Sephadex G-100 gel filtration.The factor was a single poly-peptide with a molecular weight of 52.5kd determined by SDS-PAGE.The heat-table toxinpossesses hemolytic,enterotoxic and cytolytic activities.The hemolytic activity on human ery-thocytes was 3.81×10^(?) HU/mg,CD_(50) for Vero cell was 0.26μg.The LD_(50) for crucian carpand mice was 4.44μg and 3.58μg respectively.The toxin was neutralized py homologous anti-bodies.The toxin shows unique characteristics as compared with other known bacterial toxinstherefore the authors propose to name the toxin'hec'toxin.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第6期432-438,共7页
Acta Microbiologica Sinica
关键词
嗜水气单胞菌
溶血性
毒素
Aeromonas hydrophila
he toxin
Hemolytic activity
