摘要
自1965年以来,我国对产气杆菌、乳酸菌、攻瑰暗黄链霉菌、玫瑰红链霉菌及游动放线菌葡萄糖异构酶(EC5.3.1.5)的生产条件、诱变育种和固定化作了很多研究,用戊二醛交联游动放线菌细胞制成的固定化酶已批量生产。嗜热菌有许多优点,如高温培养周期短、杂菌污染少、节省冷却水。
In screening high producer of glucose-isomerase(GI),thermophilic Streptomycesstrain 5438(activity of GI,50u/ml broth)was chosed as an original strain,whichwas isolated from 1637 soil samples in Hainan Island of China.After repeated treatment with UV,MNNG and natural selection,the mutantstrain M1033-9 was selected.Its activity of GI reached 939u/ml broth which asfinally composed contains:wheat bran 5%(w/v),soybean cake powder 1.2%,MgSO_4·7H_2O 0.1%,K_2HPO_4·3H_2O 0.1%,CoCl_2·3H_2O 0.024%,initial pH 6.7(producing condition of GI,pH6.7—7.5),on 5000L fermentor at 40±1℃(producingcondition of GI,37—40℃)and aerating rate 1∶0.2—0.3(producing condition ofGI,1∶0.1—0.8)for 60 hours.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第5期380-382,共3页
Acta Microbiologica Sinica
基金
“六五”攻关项目
“七五”攻关项目
