摘要
高效表达霍乱毒素B亚单位的工程菌MM2菌株,在玉米浆培养基中能高效合成B亚单位,观察了吸光度(OD)、pH及B亚单位产生的动态变化。玉米浆培养基不仅成本低、制备工艺简易,而且 B 亚单位产量高,在50L 发酵罐中培养,B 亚单位产量可达40μg/mL。
Studies indicate that the cholera B subunit could be synthesized efficiently in cornsteep liquor by engineered E.coli strain MM2.We have also determined the optical density,pH and the yield of B subunit kineticly during fermentation.Corn steep liquor mediumhas advantages in low cost,simplicity in technological process and high yield of B subunitwhich can reaches 40μg/ml in 50L fermentative tank.
出处
《微生物学报》
CAS
CSCD
北大核心
1993年第3期177-181,共5页
Acta Microbiologica Sinica
关键词
霍乱毒素
工程菌
发酵
Colera toxin
Engineered bacteria
Fermentation
