摘要
从酒窖分离到一株编号为 8502-3的细菌。该菌株能以甲醇为唯一碳源和能源生长,并累积 PHB 颗粒。能以乙醇、甲胺、二甲胺、三甲胺、甲酸盐、乙醇酸、乙酸盐、乙醛酸、乙醛和蛋白胨为唯一碳源生长。有氧时,最适氮源为铵盐和硝酸盐。厌氧条件下,能以硝酸盐作为氮源生长。最适生长 pH 和温度分别为7.2和35.0℃。DNA 中 G+C 含量为65.5 mol%。它具侧生单鞭毛,在2.5%氯化钠中不能生长,不能用丙三醇、丁酸钠和天冬氨酸作为碳源生长。经鉴定认为此菌株为一新亚种,命名为查氏生丝微菌成都亚种(Hyphomicrobium zavarziaiisubsp.chengduense subsp.nov.)。
Bacterium 8502-3 was isolated from a Chinese wine-making cellar.It could utilize me-thanol as the sole source of carbon and energy and accumulated polyhydroxybutyrate in theircells.It could grow on methylamine,demethylamine,trimethylamine,ethanol,acetate,formate,glycolate,glyoxylate,acetaldehyde and peptone as the sole carbon source too.Under aerobicconditions the most suitable nitrogen source for its growth was the ammonium salts and nitrate.Under anaerobic conditions,it was able to grow with nitrate as the nitrogen source.The opti-mum pH and temperature for its growth were 7.2 and 35℃ respectively.G+C content ofDNA was 65.5mol%(Tm).Its swarmer had a single lateral flagellum.It couldnt growth in thepresence of 2.5% sodium chloride.It did not utilize glycerol,n-butyrate and aspartate as thegrowth source of carbon and named as Hyphomicrobiura zavarzinii subsp,chengduensesubsp,nov.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第3期161-166,共6页
Acta Microbiologica Sinica
关键词
生丝微菌
酿酒
新亚种
PHB-producing bacterium
Hyphomicrobium
Wine-making
