摘要
目的:测定大肠杆菌β-葡萄糖醛酸酶在不同培养基配方及不同培养时间的酶活。方法:利用对硝基酚-β-D-葡萄糖醛酸苷作为酶反应底物,在波长为405nm下比色,检测酶反应所释放的对硝基酚的量,以测定液体培养基中β-葡萄糖醛酸酶活性。研究结果表明,培养基配方2酶活性较高,对pH缓冲能力强,适合于产β-葡萄糖醛酸酶。
This experiment was conducted to determine the β-glucuronidase activity produced by E.coli in different formula of culture medium and different culture time. p-Nitrophenyl-β-D-glucuronide (PNPG) was used as substrate to determine the amount of p-nitrophenol released in the enzyme reaction with spectrometer at 405nm. It was found that β-glucuronidase activity of formula two was the highest, buffer capacity was good, it was adaptable for producing of β-glucuronidase.
出处
《中国食品学报》
EI
CAS
CSCD
2003年第4期82-85,共4页
Journal of Chinese Institute Of Food Science and Technology
