摘要
采用农杆茵(agrobacterium tumefaciens)介导法将多聚半乳糖醛酸酶(PG)反义基因导入新疆加工番茄(lycopersicon esculentum,品种代号98-75)。通过卡那霉素抗性筛选获得10株转化植株,PCR和Southern blot分析表明,其中4株为阳性植株,反义PG基因已整合到这4株加工番茄基因组中。转基因加工番茄的硬度和可溶性固形物含量高于同期对照果实;果实饱满有光泽;此4株转基因番茄果实的PG活性分别为对照番茄果实的83％、88％、63％和46％;脂氧合酶(LOX)活性下降;可溶性果胶含量在同期低于对照。Northem blot分析表明,转基因番茄果实的PG、LOX和LeEXPl基因表达水平下降。研究结果表明,转反义PG基因加工番茄果实成熟软化进程被延缓,果实品质有一定改善。
Agrobacterium -mediated transformation has been used to introduce the anti -PG gene into processing tomato (cv.98-75) . Ten transformed tomato plants were selected with kanamycin-resistant experiment. Four positive transformed tomato plants were confirmed by PCR and Southern blot. Fruit firmness and soluble solid of transgenic tomato were higher than those of control; fruit were full and lustrous; soluble pectin was lower than control at the same stage. The PG activities of transgenic tomatoes were 88%, 83%, 46%, 63% of control tomato respectively; LOX activity decreased. Northern blot hybridization showed, PG, LOX and LeEXPl mRNA amounts all decreased in transgenic tomato fruit compared with the normal tomato. Those results suggested that the process of ripening and softening in transgenic anti-PG tomato was delayed. The quality of transgenic tomato fruit was certainly improved.
出处
《中国食品学报》
EI
CAS
CSCD
2003年第4期27-32,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家杰出青年基金(No.39825118)
国家自然科学基金(No.30270934)
