摘要
目的 研究骨髓干细胞移植治疗Duchenne型肌营养不良鼠 (mdx鼠 )的效果。方法 取 4～ 5周龄昆明鼠骨髓干细胞 ,体外培养 3d ,静脉移植到 7Gyγ射线预处理 7～ 8周龄mdx鼠体内。临床监测受体鼠移植物抗宿主病 (GVHD)表现 ,并对移植 12周mdx鼠的运动功能、肌电生理、dystrophin蛋白表达情况进行检测。 结果 5只 7Gy剂量放疗mdx鼠 ,静脉移植 4 8× 10 6骨髓干细胞 ,3个月后 ,肌电图指标有了部分改善 ;10 %肌纤维表达了dystrophin蛋白。结论 静脉移植同种非同系鼠骨髓干细胞治疗mdx鼠有效 ,显示骨髓干细胞移植治疗DMD有着理想的前景。
Objective To investigate the effects of Duchenne muscular dystrophy mice(mdx) treated with stem cells transplantation.Methods The bone marrow stem cells of Kunming mice (4 to 5 weeks age) were cultured in vitro for 3 days,then injected intravenously into the mdx mice (7 to 8 weeks age),which were preconditioned with 7 Gy γ ray.The clinical scores of graft versus host disease(GVHD)were assessed and recorded from the mice per week after transplantation.12 weeks after being transplanted,the locomotion function,muscle electrophysiologic items and dystrophin expression of the mdx mice were studied.Results Three months after bone marrow stem cells transplantation,about 10% muscle cells of all transplanted mice had expression of dystrophin protein.About the electromyographic items,the durations of mild contraction of transplanted mdx mice [(5.46±0.34) ms],as compared to those [(4.42±0.31) ms] of negative control mdx mice,showed longer nearly up to those [(6.36±0.53) ms] of the positive control C57 mice ( P <0.05).The greatest contraction amplitudes of transplanted mdx mice [(3170±876) μV],comparing to those [(1190±334) μV] of the negative control mdx mice,were shown much more nearly to those [(7100±1536) μV] of positive control C57 mice ( P <0.05).Conclusions 12 weeks after mdx mice transplanted with allogenic bone marrow stem cells,the electromyographic items of the mice are improved,and there are dystrophin protein expression on part of the skeletal muscle cells.It suggests that there are ideal prospect for DMD by treating with bone marrow stem cells.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2003年第6期443-446,共4页
Chinese Journal of Neurology
基金
国家自然科学基金资助项目 (3 0 170 3 3 7
3 9870 80 4)
卫生部临床学科重点项目 (2 0 0 13 2 1)
教育部骨干教师资助项目(2 0 0 0 2 3 49)
广东省自然科学基金资助项目 (970 0 61)
中山医科大学2 11工程重点学科建设课题资助项目 (9814 9)
