摘要
目的 :探讨养血止痒片对 2 ,4 二硝基氟苯 (DNFB)所引起的小鼠Ⅳ型变态反应的抑制作用。方法 :NIH雌性小鼠 3 0只 ,在腹部去毛 ,面积约 3cm2 ,涂 0 .5 %DNFB 2 5 μL致敏 ,第 5天在左耳背面涂 0 .2 5 %DNFB 2 5 μL诱发皮炎 ,制得小鼠DNFB变应性接触性皮炎模型 ,分成养血止痒片 (3 .0g·kg 1 )组、醋酸泼尼松 (2 4.0mg·kg 1 )组和 0 .9%氯化钠注射液 (2 0 .0mL·kg 1 )组 ,每组 10只 ,均于致敏后灌胃给药 ,bid ,共给药 5d ,观察小鼠耳部的炎症情况。结果 :与 0 .9%氯化钠注射液组比较 ,养血止痒片组与醋酸泼尼松组小鼠耳部炎症抑制明显 ,单、多形核细胞数均明显减少 (P <0 .0 5 ) ,病理改变明显减轻。结论 :养血止痒片可明显抑制DNFB引起的小鼠Ⅳ型变态反应 ,是其治疗湿疹的机制之一。
Objective:To probe into the effect of Yangxuezhiyang tablets (YXZYT) on type IV allergic reaction induced by 2,4 dinitrofluorobenzene (DNFB) in mice. Methods:30 female NIH mice served as the subjects of the study. An area of round 3 cm 2 on the abdomen of each mouse was dipilated and smeared with 25 μL of 0.25% DNFB for sensitization. 5 days later, the dorsal surface of the left ear of each mouse was smeared with 25 μL of 0.25% DNFB to induce dermatitis, which was the model of DNFB allergic contact dermatitis in the mouse. The animals were then divided into 3 equal groups. Mice of group(1) were given each(3.0 g·kg ) of YXZYT, those of group(2) were given each (24.0 mg·kg 1 ) of prednisone acetate and those of group(3) were given each 20 mL·kg 1 of normal saline. All drugs were given by gastrogavage, b.i.d., for 5 consecutive days. The weight difference between the right ear and left ear and the histopathologic changes in the affected ear of each mouse were examined. Results:As compared with changes in animals of group(3) treated with NS, the inflammatory response in the affected ears of mice treated with YXZYT [group(1)] and predicone acetate [group(2)] was dramatically inhibited. There was a sharp decrease in the numbers of monoclear and polymorphonuclear cells ( P < 0.05) , signifying a definite alleviation of pathologic changes. Conclusion:YXZYT was shown to exert a distinct inhibitory effect on the type IV allergic reaction in mice induced by DNFB. It seems to be one of the underlying mechanisms of the effect of YXZYT in the treatment of eczema.
出处
《医药导报》
CAS
2003年第12期838-840,共3页
Herald of Medicine
关键词
养血止痒片(组方:熟地、当归、白芍、丹皮、生牡蛎、甘草等)
皮炎
变态反应
Ⅳ型
湿疹
Yangxuezhiyang tablet(composed of:Radix Rhmanniae Preparata,Radix Angelicae Sinensis,Radix Paeoniae Alba,Cortex Moutan, Concha Ostreae, Radix Glycyrrhizae, et al )
Dermatitis
IV allergic reaction
Eczema
