摘要
目的 :探讨移植肝一氧化氮合酶的表达与急性排斥反应间关系及其细胞定位。方法 :应用近交系 BN至 Lew大鼠原位肝移植急性排斥反应及同基因 ( Lew- Lew)肝移植动物模型 ,用免疫组化法检测移植肝组织中诱导型一氧化氮合酶 ( i NOS)的表达。同时观察 i NOS抑制剂氨基胍和免疫抑制剂 FK5 0 6对肝移植术后急性排斥反应的影响。结果 :在急性排斥组 i NOS表达强阳性 ,与氨基胍组、FK5 0 6组及同基因对照组比较差异显著。结论 :在大鼠原位肝移植发生急性排斥反应时 i N-OS增高程度与排斥反应的强度有明显关系。氨基胍、FK5 0 6可以抑制 i NOS的表达。抑制 i
Objective:To explore the effects of inducible nitric oxide synthase (iNOS) in the process of acute rejection.Methods:BN to Lew rat orthotopic liver transplanatation models were established in the experiment to detect the expression of iNOS in liver tissues with acute rejection.The influence between aminoguanidine/FK506 and the acute rejection were studied.Results:iNOS expressed strongly in the process of acute rejection.iNOS in the aminoguanidine or FK506 group or Lew Lew group were significantly lower than those in acute rejection group.Conclusion:The application of aminoguanidine and FK506 inhibite the expression of iNOS.The iNOS inhibitor may prevent or reduce the occurrence of acute tissue rejection.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第6期792-795,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题 (990 5 6 2 - 1 )
关键词
一氧化氮合酶
肝移植
免疫抑制剂
移植物排斥
大鼠
Nitric oxide synthase
Liver transplantation
Immunosuppressive agents
Graft rejection
