摘要
目的 :开发吉林双阳梅花鹿的基因资源 ,在分子水平上揭示鹿药材的药理作用。方法 :从鹿脾脏细胞中提取总 RNA,逆转录成 c DNA,构建 c DNA质粒文库 ,从中克隆 2个管家基因并进行序列分析。结果 :成功克隆的管家基因的一个核酸序列及其对应的氨基酸序列与人、大鼠和小鼠蛋白酶体 α3型亚单位 c DNA序列及对应的编码氨基酸序列高度同源 ;另一个管家基因的序列及对应的氨基酸序列与人、大鼠和小鼠转录激活因子 - 4部分 c DNA序列及对应的编码氨基酸序列高度同源。结论 :新发现的双阳梅花鹿蛋白酶体α3型亚单位部分 c DNA序列和转录激活因子 - 4部分c DNA序列 ,已在 Genbank中登录 ( BG67371 3,BG67371 4 )。
Objective: To develop the gene resources of Jinlin Shuangyang Sika deer and reveal the pharmacological functions of deer herbs on the molecular level. Methods: The total RNA was isolated from deer spleenocytes and then transcripted into cDNA reverselly. The cDNA library was constructed. Two housekeeping genes were cloned and analyzed from cDNA library. Results:One cDNA clone and it′s corresponding amino acid sequences in deer from successful cloned housekeeping genes shared high homology with a group of partial cDNA sequence encoding proteasome subunit alpha type 3 of human, rat, mouse and their corresponding amino acid sequences, respectively. The other housekeeping gene sequence and its corresponding amino acid sequence in deer shared high homology with a group of partial cDNA sequences encoding activating transcription factor 4 of human, rat, mouse and their corresponding amino acid sequences, respectively. Conclusion:Two novel partial cDNA clones encoding Shuangyang Sika deer proteasome subunit alpha type 3 and activating transcription factor 4 have been deposited in Genbank under accession number BG673713 and BG673714,respectively.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第6期713-718,共6页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题 (30 70 70 5 )
