期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

植物次生代谢途径的遗传操作 被引量：1

Genetic modification of secondary metabolite biosynthesis in higher plants: a review
原文传递
导出
摘要 植物次生代谢产物种类极其丰富,是人类的宝贵资源,这些产物及其合成途径相关酶具有空间特异性分布的特征。植物次生代谢途径的调控是个复杂的过程,受代谢产物水平、多酶复合物相互作用等多种因素的影响。通过遗传操作改造代谢过程,调控产物在植物体内的含量,是一条切实可行和具有广阔发展空间的途径。目前,改造植物次生代谢途径可以采取单基因操作和多基因操作两种策略进行。 Plants provide an immense reservoir of natural secondary metabolites. Secondary metabolites and those involved enzymes accumulate in various compartments in specific plant tissues. The biosynthesis of diverse groups of secondary metabolites is often complicated, tightly controlled via network interconnections, metabolite levels, metabolite channeling and multi-enzyme complexes, and so on. Secondary metabolite profiles could be genetically altered by two strategies, i.e. single gene modification and multiple gene modification; which thus has opened a feasible and prospective platform for secondary chemicals production in plant.
作者 王晓云 夏循礼 黄凤兰 张寿文
机构地区 江西中医学院药学院江西省中药种质资源工程技术研究中心 内蒙古民族大学生命科学学院
出处 《生物工程学报》 CAS CSCD 北大核心 2012年第10期1151-1163,共13页 Chinese Journal of Biotechnology
基金 江西省教育厅科学技术研究项目(No.GJJ11542) 国家十二五科技支撑计划(No.2011BAI04B01) 江西中医学院博士启动基金课题(No.530046)资助~~
关键词 次生代谢产物 途径 调控 单基因操作 多基因操作 secondary metabolite, biosynthesis, regulation, single gene manipulation, multiple gene manipulation
分类号 Q943 [生物学—植物学]
  • 相关文献

参考文献23

  • 1Maqbool SB, Christou P. Multiple traits of agronomic importance in transgenic indica rice plants: analysis of transgene integration patterns, expression levels and stability. Mol Breeding, 1999, 5(5): 471-480.
  • 2Peach C, Velten J. Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol Biol, 1991, 17(1): 49-60.
  • 3Ryan MD, King AMQ, Thomas GP. Cleavage of foot-and mouth disease virus polyprotein is mediated by residues within a 19 amino acid sequence. J Gen Virol, 1991, 72(Pt 11): 2727-2732.
  • 4St-Pierre B, Vazquez-Flota FA, De Luca V.Multicellular compartmentation of Catharanthus roseus alkaloid biosynthesis predicts intercellular translocation of a pathway intermediate. Plant Cell, 1999, 11(5): 887-900.
  • 5Liao ZH, Chen M, Gong YF, et al. Isoprenoid biosynthesis in plants: pathways, genes, regulation and metabolic engineering. J Biol Sci, 2006, 6(1): 209-219.
  • 6Irmler S, Schroder G, St-Pierre B, et al. Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72al as secologanin synthase. Plant J, 2000, 24(6): 797-804.
  • 7李凤岚,邱德有,马小军,刘敏,许德荣.利用RNAi抑制曼地亚红豆杉细胞紫杉烷14β-羟基化酶基因的表达[J].中国生物工程杂志,2009,29(5):55-60. 被引量：5
  • 8Ye XD, Al-Babili S, Kloti A, et al. Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science, 2000, 287(5451): 303-305.
  • 9Guillet G, Poupart J, Basurco J, et al. Expression of tryptophan decarboxylase and tyrosine decarboxylase genes in tobacco results in altered biochemical and physiological phenotypes. Plant Physiol, 2000, 122(3): 933-944.
  • 10Nawrath C, Poirier Y, Somerville C. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Proc Natl Acad Sci USA, 1994, 91(26): 12760-12764.

二级参考文献39

  • 1胡新玲,徐妙云,刘德虎,邱德有.紫杉烷14β-羟基化酶基因片段的克隆及其植物表达载体的构建[J].分子植物育种,2006,4(2):243-250. 被引量：6
  • 2Ketchum R E B, Croteau R B. Recent progress toward an understanding of taxol biosynthesis in plant cell cultures. In: Ageta H, Aimi N, Ebizuka Y, et al. Towards nature medicine research in the 21st century, the International Symposium on Natural Medicines. Kyoto, Japan, 1997. Japan: Elsevier Science B V, 1995, 339- 348
  • 3Rowinsky E K, Cazenave L A, and Donebower R C. Taxol: a novel investigational antimicrotubule agent. J Nat Cancer Inst, 1990, 82( 15 ) : 1247 - 1259
  • 4Holmes F A, Waiters R S, Theriault R L, et al. Phase II trial of Taxol, an active drug in the treatment of metastatic breast cancer. J Natl Cancer Inst, 1991, 83(24) :1797 - 1805
  • 5Wani M C, Taylor H L, Wall M E, et al. The isolation and structure of Taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc, 1971, 93(9) :2325 -2327
  • 6Ronald L, Hanson S, John M, et al. Site-specific enzymatic hydrolysis of taxanes at C-10 and C-13 J Biol Chem, 1994, 269 (35) :22145 - 22149
  • 7Vongpaseuth K, Roberts S C. Advancements in the understanding of paclitaxel metabolism in tissue culture. Curt Pharrn Biotechnol, 2007, 8 (4) :219 - 236
  • 8Jennewein S, Rithner C D, Williams R M, et al. Taxoid metabolism: taxoid 14β-hydroxylase is a eytochrome P450- dependent monooxygenase. Arch Biochem Biophys, 2003, 413 (2) : 262 - 270
  • 9Kim C H, Kim K I, Chung I S. Expression of modified green fluorescent protein in suspension culture of Taxus cuspidata. J Micro Biotech, 2000, 10 ( 1 ) :91 ~ 94
  • 10Ketchum R E B, Croteau R B. Stable transformation and long-term maintenance of transgenic Taxus cell suspension cultures. Plant Cell Rep, 2007, 26(7) :1025 -1033

共引文献33

同被引文献18

引证文献1

二级引证文献5

生物工程学报
2012年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部