摘要
弧菌病(vibriosis)是我国南方沿海地区养殖真鲷(Pagrus major)中经常发生的主要细菌性疾病,该病的暴发性流行给整个真鲷养殖业造成了巨大的经济损失.在明确其病原菌是最小弧菌(Vibrio mimicus)基础上[1],对该菌的致病机理进行了深入的研究,并取得了重要的进展.
Vibriosis, caused by Vibrio mimicus, became a major cause of serious economic losses in the farming of red sea bream along the coast of southern China. It has been demonstrated that the pathogenicity of V. mimicus for red sea bream is due to the production of a heat-labile exotoxin. In the present paper, a dot immunobinding assay (DIA) to detect the toxin produced by V. mimicus was developed. The lowest concentration of the toxin the DIA could detect was 0.025μg·mL-1. The DIA could detect the toxin in the supernatant of liver and spleen of the diseased red sea bream Pagrus major, even if the supernatant was diluted by 1000 times. It was concluded that the DIA would be useful for rapid and effective detection of the toxin in large samples.
出处
《水产学报》
CAS
CSCD
北大核心
2003年第6期606-609,共4页
Journal of Fisheries of China
基金
中国科学院知识创新工程资助项目(KSCX2-1-04)
"十五"国家高技术研究发展计划(863计划)资助项目(2001AA622050)
广东省"科技创新百项工程"资助项目(99806201G)
关键词
海水养殖
真鲷
弧菌病
病原菌
外毒素
点酶法
检测技术
Pagrus major
vibriosis
Vibrio mimicus
Vm-Pm toxin
dot immunobinding assay(DIA)
