摘要
目的 :了解熄风开窍、通腑化痰、清热熄风通络、活血化瘀等不同治则方药对脑缺血再灌注大鼠脑组织能量代谢的影响。方法 :用大脑中动脉闭塞 (MCAO)法复制大鼠局部脑缺血再灌注模型 ,分别灌服熄风开窍合剂、通腑合剂、羚蝎胶囊、中风回春丸 ,检测脑组织 ATP酶的变化。结果 :与模型组相比 ,羚蝎胶囊组、中风回春丸组 Na+ K+ ATP酶、Ca2 + ATP酶和 Mg2 + ATP酶活性均显著升高 ,熄风开窍合剂组 Ca2 + ATP酶、Mg2 + ATP酶活性均显著升高 ,而通腑合剂组各 ATP酶升高不显著。结论 :以清热熄风通络、活血化瘀和熄风开窍治则为主立方的方药具有提高大鼠局部脑缺血再灌注模型脑组织 ATP酶活性的作用。
Objective: To investigate the influence of different treating principles and herbal formulas for extinguishing wind and opening the aperture(熄风开窍), dredging Fu organs and dispersing phlegm(通腑化痰), clearing away heat, extinguishing wind and dredging the meridians(清热熄风通络) and for activating blood circulation to remove stasis(活血化瘀) on energy metabolism of brain tissue in rats with focal cerebral ischemia/reperfusion(I/R) injury. Methods: Rat model of cerebral I/R injury was established by the middle cerebral artery occlusion. Various groups of the rats models were respectively fed with windextinguishing and apertureopening decoction(熄风开窍合剂,WEAOD), Fu organdredging decoction (通腑合剂,FODD), antelope horn and scorpion capsule (羚蝎胶囊,AHSC), stroke healing pill (中风回春丸,SHP), and ATPase activity in brain was observed in cerebral I/R model rats. Results: Activities of Na+K+ATPase, Ca 2+ATPase, Mg 2+ATPase were higher in group of AHSC and group of SHP than those in control group. Compared with control group, activities of Ca 2+ATPase and Mg 2+ATPase were increased significantly in WEAOD group. Significant difference was not found in ATPase activity between control group and FODD group. Conclusion: The herbal formulas guided by the treating principles of clearing away heat, extinguishing wind and dredging the meridians, activating blood and dispering phlegm, and extinguishing wind and opening the aperture have the function of regulating the cerebral ATPase activity in rats with focal cerebral I/R injury.
出处
《中国中西医结合急救杂志》
CAS
2003年第6期366-368,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
上海市黄浦区科委资助课题 ( 2 0 0 2 HCG 15 )
关键词
方药
大鼠
局部脑缺血
再灌注
脑组织
能量代谢
ATP酶
different treating principles
extinguishing wind and opening the aperture
dredging Fu organs and dispersing phlegm
clearing away heat, extinguishing wind and dredging the meridians
activating blood circulation to remove stasis
cerebral i
