摘要
目的 :利用SO2 等离子体对聚乳酸和聚醚酯膜进行表面改性 ,研究人类涎腺细胞系HSG细胞在生物材料上的生长。方法 :材料的表面性质通过表面接触角和X射线光电子能谱 (XPS)进行表征。细胞形态与生长情况则通过倒置显微镜观察和MTT检测来评估。结果 :用SO2 等离子体改性后 ,膜表面接枝上磺酸基团 ,亲水性明显增强 ;在几种材料中 ,用SO2 等离子体改性后的聚醚酯膜更适合HSG细胞的粘附生长。结论 :用SO2 等离子体改性后的聚醚酯材料可能用于人造涎腺的支架材料。
Objective: To investigate the growth of human salivary gland (HSG) cells on the modified biomaterials. Methods: Surface modification of polylactic acid (PLA) and poly(ethylene oxide terephtalate)/poly (butylene terephtalate) (PEOT/PBT) membranes were accomplished by SO2 plasma treatment. Surface properties of materials were characterized by contact angle and X-ray photoelectron spectrum (XPS) analyses. Cell morphology and growth were observed under inverted microscope and evaluated by MTT test, respectively. Results: Sulfonic groups were chemically attached to the membrane surfaces after SO 2 plasma treatment, and the hydrophilicity of the membranes was remarkably improved. The modified PEOT/PBT membrane was found to be the most suitable for the adhesion and growth of HSG cells. Conclusion: The PEOT/PBT polymer modified by SO2 plasma may have a potential use as the scaffords for artificial salivary gland.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2003年第6期543-547,共5页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金资助项目 ( 3 0 170 2 5 4)
北京市科技项目 (H0 10 910 0 10 113 )资助项目
关键词
聚乳酸
聚醚酯
S02等离子体
HSG细胞
细胞生长
生物材料
表面改性
Adhesion
Biomaterials
Cell culture
Cell membranes
Growth kinetics
Hydrophilicity
Polyethylenes
X ray photoelectron spectroscopy
