摘要
目的 :探讨阴沟肠杆菌分布特征及产 Amp C酶菌和非 Amp C酶菌的耐药性。方法 :对临床分离的 158株阴沟肠杆菌分布科室、感染部位及对 16种抗生素耐药性进行分析 ,并通过酶粗提物头孢西丁三维试验结合 PCR法检测 Am p C酶。结果 :标本来源主要为患者的痰液、尿液、创口分泌物等 ,科室以重症监护室为多 ,感染部位以呼吸道为主 ,耐药性较高的抗生素为头孢西丁、头孢噻肟、头孢曲松等 ,158株阴沟肠杆菌中产 Amp C酶菌株共 3 3株 ,产 Amp C酶阳性率占总菌株数 2 0 .9% ,产 Amp C酶菌株对各种抗生素的耐药率比不产 Amp C酶的明显增高。结论 :阴沟肠杆菌的耐药与产 Amp C酶有关 。
Objective:To investigate the drug-resistance and distribution of 158 strai ns Enterobacter cloacae.Methods:Drug-resistance test to st rain s Enterobacter cloacae was detected in 16 kinds of antibiotics.The drug sens itiv ity tests was performed by the method of Kirby-Bauer paper-diffusion with the s tandard of NCCLS.AmpC enzyme was examined by cefoxitin three dimension test and PCR Amplification of ampC structure gene were studied.Results: The main sources of specimen were sputum,urine and wound secretion.The respirat ory tract was a major attribute of the development of Enterobacter cloacae. Enterobacter cloacae strain emerged mostly in the intensive care unites.The drug resi stance to cefoxitin,cefotaxime and ceftriaxone were high.Results: Of PCR amplification revealed that of 158 Enterobacter cloacae strains,33 strains had ampC structure gene which account for 20.9%.The drug resistance of Enterobacter cloacae p roducing AmpC enzyme were significantly higher than those of non-producing AmpC enzyme.Conclusions:The drug-resistance of Enterobacter clo acae was associated with AmpC enzyme.The best choice of treatment was imipenem .
出处
《中国微生态学杂志》
CAS
CSCD
2003年第6期331-332,共2页
Chinese Journal of Microecology
基金
国家自然科学基金资助 (项目编号 :39970 6 75)
