摘要
目的 :了解低聚果糖 (FOS)体内外对肠道菌双歧杆菌、类杆菌和肠杆菌科菌的增殖作用。方法 :FOS 1g/ (kg· bw· d) ,灌服 (ig)小鼠 ,连续 14 d后 ,取粪便 ,选择性培养基平板菌落计数法测各菌群。体外试验中 ,按 1%的 FOS添加到各人肠道分离菌培养液中 ,培养 2 4h后测其吸光度 A值和 p H值变化。结果 :ig FOS的小鼠肠道双歧杆菌和肠杆菌数量分别是 9.16± 0 .67和 8.3 3± 0 .70 (log10 N CFU/ g) ,高于对照组(P<0 .0 5)。 FOS体外对各肠道分离菌均有增殖作用 ,依大小分别是双歧杆菌、类杆菌和肠杆菌 ,其 A值分别增加了 0 .8～ 1.192、0 .80 2和 0 .198～ 0 .461,其 p H值分别降低了 1.5～ 1.68、1.2和 0 .2～ 0 .58。结论 :FOS体内外有相对选择性增殖双歧杆菌的作用 。
Objective:To study the effects of Fruc tooliosaccharid(FOS) on intestinal flora.Methods:The mice were fed FOS 1 g/(kg·bw·d) for 14 days. The feacal flora were assaye d.In vitro,FOS(1%) was added to every cultures of human intestinal bacterium .Their A and pH dates were assayed after 24 hours.Results:The counts of Bifidobacterium and Enterobacteriac eae flora were increased than the control group (P<0 05) In vitro ,the counts of Bifidobacterium、Enterobacteriaceae and Bacteroide were in creased.Their A date were 0 8～1 192、0 802 and 0 198～0 461,respect ively.Conclusion:The FOS can increase the counts of Bifidobacterium b y relative selectivity in vivo and in vitro,and can regulate the counts of Enterobacteriaceae and Bacteroide,too
出处
《中国微生态学杂志》
CAS
CSCD
2003年第6期321-322,共2页
Chinese Journal of Microecology
基金
河南省卫生厅基金资助 (编号 980 2 6 )
