摘要
〔目的〕建立灵敏、准确、特异、试剂稳定的碳酸酐酶自动生化分析法测定血清锌。〔方法〕用三氯醋酸沉淀血清中蛋白质 ,释放出来的锌离子复活脱辅基的碳酸酐酶 ,以醋酸对硝基酚作为酶促反应底物进行测定。〔结果〕线性范围达 61.2 μmol/L ,回收率 96.5 %～ 10 4.3 % ,批内和批间变异系数分别小于 3 .0 %与 4.2 %。本法 (X)与原子吸收分光光度法 (Y)比较具有良好的相关性 ,Y =1.0 1X + 0 .15 ,r =0 .988。Cu2 + 、Fe2 + 、Mn2 + 各 2 0 0 μmol/L对锌测定均无影响 ;Co2 +10 μmol/L、胆红素 3 42 μmol/L、甘油三酯 15 .0mmol/L、血红蛋白 5g/L以下对本法测定结果也无干扰。〔结论〕该法具有特异、准确、灵敏、试剂稳定等优点 ,适合于生化分析仪检测。
睴bjective〕To establish a sensitive,accurate,specific automated enzymatic method of determination of serum zinc with stable reagents by means of carbonic anhydrase(CA) reactivation .〔Methods〕 Serum proteins were precipitated with trichloroacetic acid (TCA) solution and the supernatant fraction was used to reactivate apoCA to catalyze p-nitrophenyl acetate as a substrate. 〔Results〕 The linearity was up to 61.2μmol/L. The recoveries ranged from 96.5% to 104.3% .The within run and between run CV was lower than 3.0% and 4.2% respectively. Comparing this method(X) with AAS(Y),we obtained the regression equation Y=1.01X+0.15, r =0.988. The assays showed no interference from as much as 200μmol/L of Cu 2+、Fe 2+、Mn 2+respectively,10μmol/L of Co 2+、342μmol/L of bilirubin、15.0mmol/L of triglyceride and 5g/L of hemoglobin.〔Conclusion〕 It is a simple, accurate, sensitive method with stable reagents and adapts to automatic analyzer
出处
《中国卫生检验杂志》
CAS
2003年第6期684-685,共2页
Chinese Journal of Health Laboratory Technology
基金
浙江省教育厅课题 (2 0 0 0 0 663)
