摘要
目的 :研究非小细胞肺癌 (NSCLC)肿瘤血管生成状态及其影响因素。 方法 :采用免疫组化方法研究 95例NSCLC肿瘤内微血管密度 (IMVD)及其与生物学行为的关系 ,同时分析基质金属蛋白酶 (MMP 2 ,MMP 9)、组织金属蛋白酶抑制物 (TIMP 2 )、血管内皮生长因子 (VEGF)、碱性成纤维细胞生长因子 (b FGF)、CD4 4变异体 6(CD4 4v6 )、一氧化氮合酶 2 (诱生型 ,NOS2 )、一氧化氮合酶 3(内皮型 ,NOS3)以及上皮型钙粘素 (E cad)表达状况对IMVD的影响。 结果 :NSCLC存在着活跃的新血管生成 ,IMVD与NSCLC的临床分期及淋巴结转移密切相关 ;在VEGF、b FGF、CD4 4v6、MMP 2、NOS2及NOS3阳性表达的癌组织中IMVD值显著高于阴性表达的癌组织 ,而TIMP 2、E cad阳性表达的癌组织中IMVD值显著低于阴性表达的癌组织。 结论 :IMVD的检测对于判断NSCLC的预后和淋巴结转移状态具有重要的临床应用价值 ;多种因素参与了NSCLC肿瘤血管生成的调节 ,其中VEGF、b FGF、CD4 4v6、MMP 2、NOS2及NOS3对血管生成起促进作用 ,而TIMP 2和E cad则可能起阻抑作用。
Objective: To study angiogenesis and its influencing factors in human non-small cell lung cancer(NSCLC). Methods: The values of intratumor microvessel density(IMVD) in 95 cases of NSCLC were determined and its correlation with pathological parameters was analyzed. The relationship between IMVD and expressions of MMP-2,MMP-9,TIMP-2,VEGF,b-FGF,CD44v6,NOS2,NOS3 and E-cad in NSCLC was assessed. Results: There was active tumor angiogenesis of NSCLC, and the values of IMVD were correlated with clinicopathological stages of NSCLC and lymph nodes metastasis. The values of IMVD in positive expressions of VEGF, b-FGF, MMP-2, TIMP-2,CD44v6,NOS2 and NOS3 were significantly higher than that in negative expressions, opposed to the expression of TIMP-2 and E-cad. Conclusion:IMVD is a valuable marker for evaluating stages and metastasis of NSCLC. MMP-2, TIMP-2, VEGF, b-FGF, CD44v6, NOS2, NOS3 and E-cad may take part in the regulation of angiogenesis in NSCLC.
出处
《医学研究生学报》
CAS
2004年第1期60-61,64,共3页
Journal of Medical Postgraduates
