摘要
目的 :将用于核物理结构分析的磁共振谱学用于新发现物 -机体低氧环境应激肽的指纹区信息研究 ,探索其质、量与高原病的发生、发展和转归的相关关系。方法 :在液态二次离子质谱、反相高效液相色谱和薄层层析扫描的基础上 ,对模拟高原环境 (减压低氧法 )的Wistar大鼠和临床急进高原病患者分别取血液进行核磁共振氢谱(NMR1H)实验 ,对所获表达 ;特征进行归属鉴定。结果 :14 3只Wistar大鼠的血清在特定波长处有特征吸收峰 ;71只实验组大鼠中有 6 9只呈现高表达 ,72只对照组大鼠中仅有 2只有轻微表达 ,两组比较相差显著 ,P <0 0 1。高原病组6 5例中 ,有 5 8例化学位移高表达 ,5例程度略低 (中度表达 ) ;健康对照组仅 2例有低表达 ,两组比较有显著性差异 ,P <0 0 1。高原动物实验与临床病例组血样在NMRH谱图均有特征化学位移 (ppm)的表达 ,r=0 92。结论 :在急进高原时有高原病易感者存在 ,在该类人群的体液中发现有低氧环境相关物质 -应激肽的存在。深入探索其结构与功能 ,为高原病易感者筛选提供谱学研究基础特征。
Objective: To explore the relation between stress peptide and the occurrence, development and outcome of altitude diseases by using nuclear magnetic resonance (NMR) spectroscopy. Methods: On the basis of liquid secondary ionic mass spectrogram, reverse high performance liquid chromatogram and thin layer chromatogram, NMR hydrogen spectrum experiment was performed to get blood s amples from Wistar rats under simulative altitude environment and from patients with acute altitude diseases. Results: Specific absorption peak could be observed at special wavelength in all the 143 rats. Sixty two of 71 experimental rats expressed high, while only 2 of 72 control rats slightly expressed. Of 65 patients with acute altitude diseases, 58 showed high and 5 showed moderate chemical shift expression, however, only 2 in healthy control group showed low expression. Diagnostic chemical shift expression (r=0 92) could be observed in both Wistar rats and patients with altitude diseases. Conclusion: The stress peptide is particular to altitude disease susceptible people, beaaring unique property of hypoxia environment. The research of the structure and characteristics of peotide can provide a new approach for altitude disease epidemiological research.
出处
《西南国防医药》
CAS
2003年第6期593-596,共4页
Medical Journal of National Defending Forces in Southwest China
基金
四川省自然基金重点课题 NO :0 0 0 2 2 1
关键词
低氧应激肽
氢谱信息
高原病
机体
诊断
altitude disease, hydrogen spectrum, stress peptide, hypoxia.
