摘要
目的评价RT-PCR法检测碳酸酐酶Ⅸ(MN/CAⅨ)基因在肾癌组织的表达的可靠性。方法以5例肾癌和5例正常肾组织为研究对象,提取总RNA,两步法进行RT-PCR反应,用MN/CAⅨ的特异性引物扩增,2%琼脂糖凝胶电泳后观察并测序。结果经过测序证实5例肾癌组织的RT-PCR产物中有4例可见特异的MN/CAⅨ条带,而正常肾组织的RT-PCR产物均无相应的MN/CAⅨ条带。结论RT-PCR法检测MN/CAⅨ在肾癌组织中的表达是准确、可靠的。
Objective To evaluate the reliability of reverse transcription (R T) -PCR in detecting the expression of the carbonic anhydrase Ⅸ(MN/CAⅨ) gene in renal cell carcinoma (RCC). Methods MN/CAⅨwas examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAⅨwas designed and two-step RT-PCR of the ex-tracted total RNA performed. The products was examined by 2% agrose electrophoresis and identified by sequence nanalysis. Results Specific bands of MN/CAⅨwere found in RT-PCR products of 4 case of RC C tissues, whereas could not be seen in normal renal tissues. Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第1期99-101,共3页
Journal of First Military Medical University
