摘要
目的 探讨烧伤患者营养支持的有效途径。方法 分别利用免疫沉淀扣除法、间接ELISA法及荧光分光光度法检测肠道喂养与肠外营养烫伤动物模型的骨骼肌中 19S调节复合体的活性和蛋白表达水平及蛋白质降解速率的变化。结果 与肠外营养相比 ,肠道喂养能明显降低烫伤大鼠骨骼肌中 19S调节复合体活性和表达水平 ,减少骨骼肌蛋白质分解。结论 早期的肠道喂养 ,可以显著抑制 2 6S蛋白酶复合体系统的活化 ,从而在整体上降低骨骼肌蛋白质分解代谢 ,可能有助于烧伤患者的代谢调理。
Objective To study the effectrve approach of the nutrition support in burn patients. Methods The means of immuno-precipitation-deduction,ELISA and fluore-photometion were used to test the change of activities,protein expression of the 19S regulator and the rate of protein degradation in skeletal muscle in scard rats with enteral feeding or parenteral nutrition. Results Compared with parenteral nutrition , enteral feeding could markedly reduce the activity and protein expression of the 19S regulator ,and the digeneration of skeletal muscle was also lower. Conclusions The early enteral feeding can distinctly inhibit the system of 26S proteasome , thereby reduce the protein degradation of skeletal muscle in scald rats,which may be benefical to the metabolic modulation of the burned patients.
出处
《中国普通外科杂志》
CAS
CSCD
2003年第12期910-913,共4页
China Journal of General Surgery
关键词
肠营养
胃肠外营养
烧伤
骨骼肌
调节复合体
ENTERAL NUTRITION
PARENTERAL NUTRITION
BURNS/ther
SKELETAL MUSSLE
REGULATOR
