摘要
目的 探讨慢性应激对大鼠血清和海马一氧化氮 (nitricoxide,NO)含量的影响及苯妥英钠对它们的效应。方法 利用强迫游泳作为应激源制作慢性应激模型 ,采用硝酸还原酶法测定各组大鼠血清和海马NO的含量。结果 对照组、应激组和应激给药组之间大鼠血清NO的含量差异无显著性 ;应激组大鼠海马NO的含量 (4 70± 15 9)nmol/g显著高于对照组 (2 34± 77)nmol/ g和应激给药组(2 0 2± 89)nmol/g (P <0 .0 1) ,后两组间的差异无显著性。结论 慢性应激对大鼠血清NO含量无影响 ,但可诱导大鼠海马NO含量升高 ,苯妥英钠可以抑制慢性应激所致海马NO的过度生成。
Objective To investigate the changes of NO levels in serum and hippocampus of rats induced by chronic stress, and to explore the effects of phenytoin on them. Methods The forced swimming was used to set chronic stress model. Using nitric acid deoxidize enzyme method, the NO levels of serum and hippocampus were measured. Results The NO levels of serum had no significant differences between each group. The NO level of hippocampus was significantly higher in stress group (470±159)nmol/g than that in control group (234±77) nmol/g and stress-DPH group (202±89)nmol/g(P< 0.01). No apparent difference was found between the control group and stress-DPH group. Conclusions The exposure to chronic stress did not cause significant change of NO levels in serum of rats, but induced excessive production of NO in hippocampus, which could be inhibited by phenytoin.
出处
《神经疾病与精神卫生》
2003年第6期417-418,共2页
Journal of Neuroscience and Mental Health
