摘要
目的 : 观察下丘脑腹内侧核损伤性肥胖大鼠在自由进食状态下脂肪合成与分解代谢的变化。方法 : 雌性 SD大鼠分为下丘脑腹内侧核损伤肥胖组 (VMH)和下丘脑腹内侧核非损伤对照组 (假手术 ) ,于下丘脑腹内侧核手术 1 w后 ,留取血样、肝脏、皮下脂肪、子宫外周和肠系膜脂肪组织以及腓肠肌分别测定生化指标、脂肪合成酶和分解酶活性。结果 : 在自由进食状态下VMH血清胰岛素水平显著高于对照组 ,而血浆游离脂肪酸显著低于对照组。大鼠肝脏微粒体甘油三酯转运蛋白 (MTP)、肝脏磷脂酰磷酸水解酶 (PAP)、苹果酸酶 (ME)、葡萄糖 - 6-磷酸脱氢酶(G6PDH)以及子宫外周脂肪组织 ME和肠系膜脂肪组织 ME、G6PDH的活性均高于对照组。与对照组相比 ,VMH组子宫外周、肠系膜脂肪组织和腓肠肌激素敏感脂酶 (HSL)活性没有变化 ,皮下脂肪组织的 HSL活性升高。在 VMH组 ,皮下脂肪组织、子宫外周脂肪组织、肠系膜脂肪组织以及腓肠肌中的脂蛋白脂酶活性均显著升高。结论 : 下丘脑腹内侧核损伤性肥胖大鼠自由进食状态下肝脏合成和转运甘油三酯能力增强 ,脂肪组织如子宫外周和肠系膜脂肪组织的脂质合成和储存增多 ;
Objective: To investigate activities of lipo-related enzymes in ventromedial hypothalamic obese rats at acute phase in fed state. Methods: Female SD rats were divided into two groups, one group received bilateral electrolytic lesions of ventromedial hypothalamus(VMH), and another one was used as sham control. Samples of blood, livers, and subcutaneous, parametric and mesenteric adipose tissues ,and gastrocnemius were collected one week after VMH lesions and sham operations in fed state. Results: Serum insulin in VMH group was higher than that in sham group, meanwhile serum free fatty acids in VMH group were lower (P<0.05). Activities of microsomal triglyceride transfer protein (MTP) in hepatocytes, and phosphatidate phosphohydrolase (PAP), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH) in liver, ME in parametric adipose and ME, G6PDH in mesenteric adipose tissue in VMH-lesioned rats were increased when compared to their sham counterparts. Activity of hormone sensitive enzyme (HSL) in parametric, mesenteric adipose tissue and gastrocnemius in VMH-lesioned rats was not changed when compared to sham group. Activity of HSL in subcutaneous adipose tissue was increased. Activity of lipoprotein lipase (LPL) in parametric , subcutaneous and mesenteric adipose tissue and gastrocnemius were enhanced significantly compared to sham group. Conclusion: At acute phase in fed state of obesity of VMH-lesioned rats, hepatic production and transportation of triglyceride in these rats increased significantly, and lipogenic metabolism and storage of triglyceride in adipose tissues such as parametric and mesenteric adipose tissues also enhanced. Meanwhile, lipolytic metabolism in subcutaneous adipose tissue and gastrocnemius increased.
出处
《营养学报》
CAS
CSCD
北大核心
2003年第4期344-347,共4页
Acta Nutrimenta Sinica
