摘要
目的 探讨不同全身放射治疗 (TBI)剂量在异基因移植中对移植物抗宿主病 (GVHD)的发生和严重程度的影响。方法 将受体大鼠随机分为A、B、C、D 4个组 ,移植当天A、B组和C、D组分别接受 2Gy和 6Gy的全身照射 ,随后给A、C 2个组移植供体鼠骨髓细胞 ,B、D组作为对照组回输等量生理盐水。观察生存期、临床GVHD表现和病理改变等。结果 A、B、C、D组生存期分别为 2 4 .6、>5 0 .0、17.8、2 0 .2d ,其中A与B、C与D及A与C组间差异有显著性意义 (t值分别为 18.3、2 .6 0、4 .4 0 ,P值均 <0 .0 5 )。临床GVHD评分A、C组分别为 2 .4、6 .8,两组差异有显著性意义 (t=7.0 0 ,P <0 .0 1)。肝脏及结肠病理检查A、C组间差异有极显著性意义 (P <0 .0 1)。结论 A组大鼠生存期比C组显著延长 ,A组GVHD发生率和严重程度比C组明显降低。
Objective To discuss the influence of different total body irradiation (TBI) doses on incidence and severity of graft versus host disease (GVHD). Methods Twenty rats were randomized to A,B,C,D groups which all received TBI. Groups A and B received 2?Gy while groups C and D 6?Gy. Groups A and C received bone marrow cells from donor rats. Groups B and D received equivalent dose of NS serving as control for comparison. Life span, clinical GVHD manifestations and pathologic changes in the colon and liver were evaluated. Results The mean life span for group A was 24.6 days as compared with 17.8 days of group C, with much longer survival in the former group (P<0.01). There were significant differences in clinical GVHD score and pathologic changes between groups A and C, with severer clinical GVHD manifestations and pathologic changes in the latter group (P<0.01). Conclusions The incidence and severity of graft versus host disease is much lower in rats which received lower total body irradiation (TBI) dose than those with higher TBI dose.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2003年第4期261-263,共3页
Chinese Journal of Radiation Oncology
