摘要
目的:研究自然杀伤(NK)细胞在单倍体相合骨髓移植中对移植物抗宿主病(GVHD)及造血重建的影响.方法:以近交系Balb/c(H-2d)×C57BL/6(H-2b)杂交F1代小鼠(H-2d/b)为供鼠、Balb/c(H-2d)为受鼠,在移植物中增加供者的外周T细胞和(或)NK细胞进行半相合骨髓移植,根据临床表现、病理检查及外周血白细胞(WBC)数,比较不同移植组GVHD发生及造血重建情况.结果:骨髓+T细胞组中90%的小鼠出现GVHD,外周血WBC平均在移植后4wk恢复正常;而骨髓+T+低浓度NK细胞组中20%小鼠出现GVHD,外周血WBC平均在移植后3wk恢复正常;骨髓+T+高浓度NK细胞组中仅10%出现GVHD,外周血WBC也平均在移植后3wk恢复正常.后两组较骨髓+T细胞组GVHD发生率显著下降(P<0.01),外周血WBC恢复时间显著提前(P<0.01).结论:在小鼠单倍体相合骨髓移植中,同种反应性NK细胞可降低GVHD发生率,促进造血重建.
AIM： To explore the effect of natural killer （NK） cell on graft-versus-host disease （GVHD） and hematopoietic reconstitution after H-2 haploidentical bone marrow transplantation in mice. METHODS： Murine model of H-2 haplotype mismatched bone marrow transplantation was established by using Balb/c （ H-2^d ） mouse as recipient, and Balb/c （ H-2^d ） × C57BL/6 （ H-2^b ） （ H-2^d/b ） mouse as donor. Lethally irradiated Balb/c （H-2^d） mice were transplanted with Balb/c （H-2^d） × C57BL/6（ H-2^b） （H-2^d/b） bone marrow plus with donor peripheral T cells and/or NK cells. GVHD and hematopoietic reconstitution were observed and evaluated by clinical manifestations, pathological changes and WBC counting. RESULTS： In the bone marrow plus with T cells group, GVHD was induced in 90% mice and the recovery of the WBC needed 4 weeks averagely; but in the group plus with low concentration of NK cells, GVHD was induced in 20% mice and the recovery of the WBC was 3 weeks averagely; and in the group transplanted with high concentration of NK cells, GVHD was induced only in 10% mice and the recovery of the WBC was 3 weeks averagely. Compared with the group transplanted only with T cells, the incidences of GVHD in the later two groups decreased obviously （ P 〈 0.01 ）, and the recovery of the WBC was in a shorter time （ P 〈 0.01 ）. CONCLUSION： In mouse H-2 haploidentical bone marrow transplantation, alloreactive NK cells can reduce the incidence of GVHD and promote hematopoietic reconstitution.
出处
《第四军医大学学报》
CAS
北大核心
2007年第5期418-420,共3页
Journal of the Fourth Military Medical University
关键词
杀伤细胞
天然
骨髓移植
移植物抗宿主病
造血重建
killer cell, natural
bone marrow transplantation
graft-versus-host disease
hematopoietic reconstitution
