摘要
AIM: To compare the difference of expression of Bcl-2 and Bax in extrahepatic biliary tract carcinoma and dysplasia, and to analyze the role of Bcl-2 and Bax proteins in the progression from dysplasia to carcinoma and to evaluate the correlation of Bcl-2/Bax protein expression with the biological behaviors.METHODS: Expressions of Bcl-2 and Bax were examined immunohistochemically in 27 cases of extrahepatic biliary tract carcinomas (bile duct carcinoma: n=21, carcinoma of ampulla of Vater: n=6), and 10 cases of atypical dysplasia.Five cases of normal biliary epithelial tissues were used as controls. A semiquantitative scoring system was used to assess the Bcl-2 and Bax reactivity.RESULTS: The expression of Bd-2 was observed in 10 out of 27 (37.0 %) invasive carcinomas, 1 out of 10 clysplasias, none out of 5 normal epithelial tissues. Bax expression rate was 74.1% (20/27) in invasive carcinoma, 30 % (3/10) in dysplasia,and 40 % (2/5) in normal biliary epithelium. Bcl-2 and Bax activities were more intense in carcinoma than in dysplasia,with no significant difference in Bcl-2 expression (P=0.1:10),and significant difference in Bax expression (P=0.038). Level of Bax expression was higher in invasive carcinoma than in dysplasia and normal tissue (P=0.012). Bcl-2 expression was correlated to Bax expression (P=0.0059). However, Bcl-2/Bax expression had no correlation with histological subtype,grade of differentiation, or level of invasion.CONCLUSION: Increased Bcl-2/Bax expression from dysplasia to invasive tumors supports the view that this is the usual route for the development of extrahepatic biliary tract carcinoma. Bcl-2/Bax may be involved, at least in part,in the apoptotic activity in extrahepatic biliary carcinoma.
AIM:To compare the difference of expression of Bcl-2 and Bax in extrahepatic biliary tract carcinoma and dysplasia,and to analyze the role of Bcl-2 and Bax proteins in the progression from dysplasia to carcinoma and to evaluate the correlation of Bcl-2/Bax protein expression with the biological behaviors, METHODS:Expressions of Bcl-2 and Bax were examined immunohistochemically in 27 cases of extrahepatic biliary tract carcinomas (bile duct carcinoma:n=21,carcinoma of ampulla of Vater:n=6),and 10 cases of atypical dysplasia. Five cases of normal biliary epithelial tissues were used as controls.A semiquantitative scoring system was used to assess the Bcl-2 and Bax reactivity. RESULTS:The expression of Bcl-2 was observed in 10 out of 27 (37.0 %) invasive carcinomas,1 out of 10 dysplasias,none out of 5 normal epithelial tissues.Bax expression rate was 74.1% (20/27) in invasive carcinoma,30 % (3/10) in dysplasia, and 40 % (2/5) in normal biliary epithelium.Bcl-2 and Bax activities were more intense in carcinoma than in dysplasia, with no significant difference in Bcl-2 expression (P=0.110), and significant difference in Bax expression (P=0.038).Level of Bax expression was higher in invasive carcinoma than in dysplasia and normal tissue (P=0.012).Bcl-2 expression was correlated to Bax expression (P=0.0059).However,Bcl-2/ Bax expression had no correlation with histological subtype, grade of differentiation,or level of invasion. CONCLUSION:Increased Bcl-2/Bax expression from dysplasia to invasive tumors supports the view that this is the usual route for the development of extrahepatic biliary tract carcinoma.Bcl-2/Bax may be involved,at least in part, in the apoptotic activity in extrahepatic biliary carcinoma.
