摘要
目的探讨bcl-2反义寡核苷酸(ASODN)对VP-16抑制人胆管癌QBC939细胞生长和诱导细胞凋亡的影响。方法合成特异性靶向Bcl-2ASODN,并将其转染QBC939细胞;四甲基噻唑蓝(MTT)试验检测Bcl-2ASODN对VP-16抑制QBC939细胞增殖的影响;流式细胞术观察Bcl-2ASODN对VP-16诱导QBC939细胞凋亡的影响。结果ASODN组细胞的存活率显著低于无义寡核苷酸(NSODN)组(P<0.05);ASODN+VP-16组细胞存活率显著低于ASODN组及VP-16组(P<0.05);ASODN组、ASODN+VP-16组和VP-16组细胞凋亡率显著高于对照组和NSODN组(P<0.05);ASODN+VP-16组细胞凋亡率显著高于ASODN组及VP-16组(P<0.05)。结论Bcl-2ASODN对VP-16抑制人胆管癌QBC939细胞生长和诱导细胞凋亡有促进作用。
Objective To investigate the effect of Bcl-2 ASODN on VP-16 induced growth inhibition and apoptosis in human bile duct carcinoma cell line QBC939. Methods Human cholangiocarcinoma cell line QBC939 was conventionally cultured. Specific target selective Bcl-2 ASODN, designed and artificially synthesized, was transfected to the cell line; MTT assay and flow cytometry （FCM） were used to study cell proliferation and apoptosis in cell line QBC939. Results MTT assay showed cell survival rate in ASODN group was significantly lower than that in NSODN group （P 〈 0.05）. The survival rate in ASODN ＋ VP-16 group was significantly lower than that in ASODN group and VP-16 group （ P 〈 0. 05 ）. FCM analysis showed that apoptosis rates in ASODN group, VP-16 group and ASODN ＋ VP-16 group were significantly higher than those in control groups （ P 〈 0. 05 ）. The apoptosis rate in ASODN ＋ VP-16 group was significantly higher than that in ASODN group and VP-16 group （P〈0.05）. Conclusions Bcl-2 ASODN can enhance the effect of VP16-induced growth inhibition and apoptosis in QBC939 cell line.
出处
《中国普通外科杂志》
CAS
CSCD
2007年第2期125-128,共4页
China Journal of General Surgery
基金
湖南省卫生厅科研基金资助项目(B2004-168)
湖南省科技厅科技计划项目(05SK3015)
