摘要
利用RT-PCR方法,从ConA诱导的鸡淋巴细胞中扩增出鸡α-干扰素基因,经同源性比较,发现家禽α-干扰素核苷酸序列之间同源性较高,而与哺乳动物的同源性较低,说明家禽α-干扰素的生物学功能与哺乳动物可能存在一定差异。
Interferon-αgene of chicken was amplified by RT-PCR from lymphocyte stimulated by ConA and sequenced subsequently.The ORF is sequenced to be582bp.The homology of the nucleotide sequence is higher compared with the sequence of avian than with mammals.The result shows there maybe some differences between the function of IFN-αof avian and mammal.
出处
《山东家禽》
2003年第11期12-13,共2页
Shandong Poultry
基金
山东省自然科学基金资助项目
