摘要
研究了咖啡酸修饰电极的制备、性质及对NADH的电催化作用。修饰电极在 0 .1mol/LPBS缓冲溶液中 (pH 7.0 )于 0 .0～ +0 .5 0V(vs.Ag/AgCl )电位范围内呈现一对氧化还原峰 ,式量电位 (E0′)为 +0 .2 5 0V(vs.Ag/AgCl)。E0′随pH增加而朝负方向移动 ,pH在 5 .0～ 8.0范围内 ,其线性回归方程为E0′=0 .6 2 33-0 0 5 996pH ,R =0 .996 9。表观电极反应速率常数 (Ks)为 12 .3s-1。电极反应的电子数为 2且有 2个质子参与。该修饰电极对NADH的氧化具有很好的电催化作用。NADH浓度在 0 .1～ 6 .0mmol/L范围内与峰电流呈现良好的线性关系。
The preparation and electrochemical properties of caffeic acid modified glassy carbon electrode and the electrochemical properties have been studied. The modified electrode gives a relative reversible redox peaks in 0.1 mol/L phosphate buffer solution (pH 7.0) over the potential range from 0.0 to 0.5 V (vs. Ag/AgCl), which the formal potential is 0.250 V (vs. Ag/AgCl). The formal potential shifts negatively as pH values increased over the range of 5.0 to 8.0. The linear regression equation is E-0'=0.6233-0.05996 pH, R=0.9969, The apparent electron transfer rate constant (K-s) was 12. 3 s(-1), The electrode reaction contain two electrons and two protons process. The caffeic acid modified electrode shows electrocatalytic activity for oxidation of dihydronicotinamide adenine dinucleotide in 0.1 mol/L pH 7.0 PBS. The peak currents were proportional to the concentrations of the dihydronicotinamide adenine dinucleotide in the range of 0.1similar to6.0 mmol/L. The mechanism of electrode reaction has been discussed in this paper.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2003年第11期1380-1384,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金 (No .2 0 175 0 2 5
2 9875 0 2 7)
电分析化学国家重点实验室资助课题
国家"九五"科技攻关重点项目 (No .96 A2 3 0 1 0 6)资助课题
关键词
咖啡酸
玻碳修饰电极
烟酰胺腺嘌呤二核苷酸
电催化氧化
电沉积
辅酶
dihydronicotinamide adenine dinucleotide
caffeic acid
chemically modified electrode
glassy carbon electrode
electrodeposited
electrocatalysis
