摘要
在200mmol/LNaCl诱导下构建诸葛菜苗期的cDNA文库,并从cDNA文库中筛选到一个新的过氧化物酶基因OvRCI,该基因cDNA全长1220bp,包含了一个1128bp的开放阅读框.通过同源分析比较及RT PCR实验等对该基因进行了分析.结果显示该基因是一个诱导型表达的基因.
New peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of Orychophragmus violaceus peroxidase gene(OvRCI, GenBank Acc. No.bankit 569146) was 1220bp and contained a 1128bp open reading frame encoding a protein of 376 amino acids. Homology analysis and molecular modeling revealed that OvRCI strongly resembled other peroxidase genes. RT-PCR analysis revealed that OvRCI was salt-inducible and its transcript level was most abundant after 24 h treatment with 200 mmol/L sodium chloride. This studies suggested that OvRCIwas a new member of the family of recently cloned peroxidase genes.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第6期1187-1190,共4页
Journal of Sichuan University(Natural Science Edition)
基金
绵阳师范学院科研基金
