摘要
本文建立了一种鱼卵中脂肪酸测定的方法 ,该方法采用CM法提取脂肪 ,室温下以氢氧化钠—甲醇酯交换法进行酯化 ,以FFAP毛细管气相色谱法进行检测 ,并对鱼卵中的主要脂肪酸进行了鉴定 ,该方法各脂肪酸的平均回收率为 90 78～ 1 0 2 45 % ,RSD为0 0 4 39～ 0 0 661 ,最小检出限为 0 2 4 8～ 0 765 μg/g ,结果表明各项指标均满足分析要求 ,具有简单、快速、准确的特点。对色谱条件进行适当优化 ,可用来分析各种动物性脂肪酸。
A method to determine fatty acids in fish egg was described in the paper.Fat was extracted with CM method,esterified with ester exchange method of potassium hydroxide-methanol solution at room temperature and analyzed by FFAP capillary columm gas chromatography.Dominant fatty acids were also identified in fish egg.Average recovery of fatty acid reached 90.78～102.45%,relative standard deviation was in the range of 0.0439～0.0661 and lowest detection limit ranged from 0.248 to 0765μg/g.If the gas chromatography conditions was optimized,the method could be used to determine different animal fatty acids.
出处
《水产学杂志》
CAS
2003年第2期35-39,共5页
Chinese Journal of Fisheries
