摘要
目的 对从贵州省农耕区的鼠类动物分离到的 2 1株莱姆病螺旋体菌株进行分子流行病学研究。方法 应用聚合酶链反应 (PCR)从 2 1株莱姆病螺旋体分离株的全基因组DNA扩出 5S～2 3SrRNA基因间隔区 ,扩增产物用限制性片段长度多态性分析 (RFLP)和核酸序列分析。结果 贵州省 2 1株莱姆病螺旋体菌株可分为两个基因型 :Borreliavalaisiana(B .valaisiana) 2 0株 ,Borreliasp1株。结论 贵州省农耕区以B .valaisiana基因型为主 ,目前B .valaisiana对人的致病性已经得到证实。
Objective To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy. Methods Polymerase chain reaction(PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer,and amplified products were analyzed by restriction fragment length polymorphism(RFLP) and nucleotide sequencing. Results There were two genospecies in the strains: 20 strains belong to Borrelia valaisiana , 1 strain is Borelia sp. Conclusion Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B.valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2003年第12期1129-1131,共3页
Chinese Journal of Epidemiology
